Cathepsin F Antibody (237208) [Unconjugated]

Cathepsin F was detected in formalin fixed paraffin-embedded sections of human heart using 8 µg/mL Mouse Anti-Human Cathepsin F Monoclonal Antibody (Catalog # MAB2075) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: 237208
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin F Antibody (237208) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cathepsin F
  Ala20-Asp484
  Accession # NP_003784
• Specificity: Detects human Cathepsin F in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, L, O, S, V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTSF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin F Antibody (237208) [Unconjugated]

• Cathepsin F
• CATSFEC 3.4.22.41
• CTSF
• EC 3.4.22

Background

Cathepsin F is a lysosomal cysteine protease that participates in protein degradation and has been implicated in tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTSF
• Uniprot:
  • NP_003784()