Cathepsin E Assay Kit (Fluorometric)

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Cell and tissue lysates, Purified Cathepsin E

Order Details

Cathepsin E Assay Kit (Fluorometric) Summary

Description
Rapid and convenient fluorometric assay to measure Cathepsin E Activity. Detection limit: 1 mU.
  • Detection method: Fluorescence (Ex/Em = 320/420 nm)
  • Applications: Detection of Cathepsin E activity in tissue and cell lysates. Determine activity of purified Cathepsin E.
Kit Type
Assay Kit (Fluorometric)
Gene
CTSE

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. MCA Standard (1 mM)
  2. Human Cathepsin E (Positive Control)
  3. CTSE Assay Buffer
  4. CTSE Lysis Buffer
  5. CTSE Substrate

Alternate Names for Cathepsin E Assay Kit (Fluorometric)

  • CATE
  • Cathepsin E
  • CTSE
  • EC 3.4.23
  • EC 3.4.23.34
  • erythrocyte membrane aspartic proteinase
  • slow-moving proteinase

Background

Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. The Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CTSE