Cathepsin D Antibody (CTD-19)

Western Blot: Cathepsin D Antibody (NB600-1295) - WB analysis of MCF7 Human breast carcinoma cells were separated on SDS-PAGE and probed with Monoclonal AB to Cathepsin D (NB600-1295) at 1/4,000. The antibody was developed with AlkPhos APA mouse Fab ads HIgG conjugate (Cat. No. A1293) and a NBT/BCIP substrate.

Product Discontinued

Cathepsin D Antibody (CTD-19) Summary

• Immunogen: Human liver cathepsin D.
• Localization: Lysosomal
• Marker: Lysosomes Marker
• Specificity: Recognizes cathepsin D (34 kDa and weaker band at 52 kDa) in immunoblotting. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTSD
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Microarray
  • Western Blot 1:1000
• Application Notes: May be useful in Electron Microscopy, as reported in literature (PMID: 21264221), and ICC/IF (PMID: 21264221; 21264228). Immunocytochemistry/Immunofluorescence and Electron Microscopy were reported in scientific literature.
• Control:

  Cathepsin D Overexpression Lysate
  MCF-7 Whole Cell Lysate

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Cathepsin D Antibody (CTD-19)

• cathepsin D (lysosomal aspartyl protease)
• Cathepsin D
• CPSD
• CTSD
• EC 3.4.23
• EC 3.4.23.5
• lysosomal aspartyl peptidase
• lysosomal aspartyl protease
• MGC2311
• neuronal 10

Background

Cathepsin D has been reported to be involved in malignant tumour progression and with prognosis of various human cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin D Antibody (NB600-1295)(4)

Publications using NB600-1295

• Wong WJ, Qiu B, Nakazawa MS et al. MYC Degradation Under Low O2 Tension Promotes Survival by Evading Hypoxia-Induced Cell Death. Mol Cell Biol 2013-07-01 [PMID: 23816886] (ICC/IF, Human)
• Miyamoto T, Kitamura N, Ono M et al. Identification of 14-3-3gamma as a Mieap-interacting protein and its role in mitochondrial quality control. Sci Rep. 2012-04-24 [PMID: 22532927] (WB, Human)
• Nakamura Y, Kitamura N, Shinogi D et al. BNIP3 and NIX Mediate Mieap-Induced Accumulation of Lysosomal Proteins within Mitochondria. PLoS One. 2012-01-26 [PMID: 22292033]
• Kitamura N, Nakamura Y, Miyamoto Y et al. Mieap, a p53-inducible protein, controls mitochondrial quality by repairing or eliminating unhealthy mitochondria PLoS One 2011-01-17 [PMID: 21264228] (EM, ICC/IF, Human)

Bioinformatics

• Gene Symbol: CTSD
• Entrez:
  • 1509(Human)
• Uniprot:
  • P07339()