Cathepsin C/DPPI Antibody [Unconjugated]

Images

 
Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) followed by ...read more
Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin C/DPPI at approximately 56 kDa (as indicated) using 50 µg/mL of Goat ...read more
Cathepsin C/DPPI was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) at 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Cathepsin C/DPPI Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human pro Cathepsin C/DPPI
Asp25-Leu463
Accession # P53634
Specificity
Detects human Cathepsin C/DPPI in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSC
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1071 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin C/DPPI Antibody [Unconjugated]

  • Cathepsin C
  • cathepsin CEC 3.4.14.1
  • Cathepsin J
  • CPPIHMS
  • CTSC
  • dipeptidyl peptidase 1
  • Dipeptidyl peptidase I
  • Dipeptidyl transferase
  • dipeptidyl-peptidase I
  • DPP1
  • DPPI
  • DPP-I
  • JP
  • JPD
  • PALS
  • PLS

Background

Cathepsin C, also known as dipeptidyl-peptidase I (DPPI), is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2). Loss of function mutations in the Cathepsin C gene result in periodontal disease and palmoplantar keratosis (3).

  1. Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1192, Academic Press, San Diego.
  2. Dahl, S.W. et al. (2001) Biochemistry 40:1671.
  3. Toomes, A.J. et al. (1999) Nat. Genet. 23:421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin C/DPPI Antibody (AF1071)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF1071 Applications Species
E Blommaert, R Péanne, NA Cherepanov, D Rymen, F Staels, J Jaeken, V Race, L Keldermans, E Souche, A Corveleyn, R Sparkes, K Bhattachar, C Devalck, R Schrijvers, F Foulquier, R Gilmore, G Matthijs Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 31036665] (Western Blot, Human) Western Blot Human
Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases J. Biol. Chem., 2016;291(16):8486-99. 2016 [PMID: 26884336] (Western Blot, Human) Western Blot Human
Cherepanova N, Shrimal S, Gilmore R Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins. J Cell Biol, 0;206(4):525-39. 0 [PMID: 25135935] (Immunoprecipitation, Human) Immunoprecipitation Human
Le QT, Min HK, Xia HZ, Fukuoka Y, Katunuma N, Schwartz LB Promiscuous processing of human alphabeta-protryptases by cathepsins L, B, and C. J. Immunol., 2011;186(12):7136-43. 2011 [PMID: 21562164] (Western Blot, Human) Western Blot Human
Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes. Blood, 2007;111(4):2339-46. 2007 [PMID: 18070985] (ICC, Human) ICC Human
Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev., 2006;20(5):543-56. 2006 [PMID: 16481467] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol CTSC
Uniprot