Recombinant Mouse Cathepsin A/Lysosomal Carboxypeptidase A His Protein

SDS-Page: Recombinant Mouse Cathepsin A/Lysosomal Carboxypeptidase A Protein [NBP2-52322] - 15% SDS Page (3 ug)

• Reactivity: Mu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Mouse Cathepsin A/Lysosomal Carboxypeptidase A His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 24-474 of Mouse Cathepsin A/Lysosomal Carboxypeptidase A
  
  

  Source:Baculovirus

  Amino Acid Sequence: APDQDEIDCL PGLAKQPSFR QYSGYLRASD SKHFHYWFVE SQNDPKNSPV VLWLNGGPGC SSLDGLLTEH GPFLIQPDGV TLEYNPYAWN LIANVLYIES PAGVGFSYSD DKMYVTNDTE VAENNYEALK DFFRLFPEYK DNKLFLTGES YAGIYIPTLA VLVMQDPSMN LQGLAVGNGL ASYEQNDNSL VYFAYYHGLL GNRLWTSLQT HCCAQNKCNF YDNKDPECVN NLLEVSRIVG KSGLNIYNLY APCAGGVPGR HRYEDTLVVQ DFGNIFTRLP LKRRFPEALM RSGDKVRLDP PCTNTTAPSN YLNNPYVRKA LHIPESLPRW DMCNFLVNLQ YRRLYQSMNS QYLKLLSSQK YQILLYNGDV DMACNFMGDE WFVDSLNQKM EVQRRPWLVD YGESGEQVAG FVKECSHITF LTIKGAGHMV PTDKPRAAFT MFSRFLNKEP YVEHHHHHH

• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: CTSA
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 52.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Mouse Cathepsin A/Lysosomal Carboxypeptidase A His Protein

• beta-galactosidase 2
• beta-galactosidase protective protein
• Carboxypeptidase C
• Carboxypeptidase L
• Cathepsin A
• cathepsin ANGBE
• CTSA
• EC 3.4.16
• EC 3.4.16.5
• GLB2
• GSL
• Lysosomal Carboxypeptidase A
• lysosomal protective protein
• PPCA
• PPGBprotective protein for beta-galactosidase (galactosialidosis)
• Protective protein cathepsin A
• Protective protein for beta-galactosidase

Background

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CTSA