Catenin alpha 1 Antibody - BSA Free

Western Blot: Catenin alpha 1 Antibody [NB120-11346] - Rat embryonic brain (S1) extract was separated on SDS-PAGE and probed with IGG FRACTION AB TO alpha-E-CATENIN (RB). The antibody was developed with HRP Anti-Rabbit IgG and an ECL substrate. Antibody dilution 1:2000.

Immunocytochemistry/Immunofluorescence: Catenin alpha 1 Antibody [NB120-11346] - MDCK Madin Darby Canine Kidney cells were fixed and permeabilized with methanol. Fixed cells were stained with 1:100 Anti-alpha-E-Catenin antibody produced in Rabbit The antibody was developed using 1:500 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat.

• Reactivity: Hu, Mu, Xp
• Applications: WB, ICC/IF, MA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Catenin alpha 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Catenin alpha 1 Antibody - BSA Free (NB120-11346) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide: EKQDETQTKIKRASQ conjugated to KLH, corresponding to amino acids 873-887 of Human Catenin alpha 1 Antibody.
• Localization: Cell Membrane and Cytoplasmic
• Specificity: Catenin alpha 1 recognizes alpha-E-catenin (102 kDa).Staining of the Catenin alpha 1 band in immunoblotting is specifically inhibited with the Catenin alpha 1 immunizing peptide (human, amino acids 873-887).
• Predicted Species: Mouse (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTNNA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Microarray
  • Western Blot 1:1000
• Application Notes: WB: Detects a band of approximately 102 kDa.
• Control:

  Catenin alpha 1 Overexpression Lysate

Reactivity Notes

This sequence is identical in mouse and Xenopus alpha-E-catenin. It is not found in alpha-N-catenin, beta-catenin, and gamma-catenin.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: IgG purified

Alternate Names for Catenin alpha 1 Antibody - BSA Free

• Alpha E-catenin
• alpha-catenin
• alpha-E-catenin
• alphaE-catenin
• cadherin-associated protein
• CAP102
• catenin (cadherin-associated protein), alpha 1 (102kD)
• catenin (cadherin-associated protein), alpha 1, 102kDa
• catenin alpha-1
• FLJ36832
• FLJ52416
• Renal carcinoma antigen NY-REN-13

Background

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTNNA1
• Entrez:
  • 1495(Human)