Catenin alpha 1 Overexpression Lysate

Images

 
Western Blot: Catenin alpha 1 Overexpression Lysate (Adult Normal) [NBL1-09575] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Catenin alpha 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Catenin alpha 1 Overexpression Lysate Summary

Description

Catenin alpha 1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201766

Accession#: NM_001903

Protein Tag: C-MYC/DDK
Gene
CTNNA1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
99.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Catenin alpha 1 Overexpression Lysate

  • Alpha E-catenin
  • alpha-catenin
  • alpha-E-catenin
  • alphaE-catenin
  • cadherin-associated protein
  • CAP102
  • catenin (cadherin-associated protein), alpha 1 (102kD)
  • catenin (cadherin-associated protein), alpha 1, 102kDa
  • catenin alpha-1
  • FLJ36832
  • FLJ52416
  • Renal carcinoma antigen NY-REN-13

Background

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CTNNA1