Catalase Antibody (OTI1B8) - Azide and BSA Free

Western Blot: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Catalase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Catalase.

Immunocytochemistry/Immunofluorescence: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Catalase.

Immunohistochemistry: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Staining of paraffin-embedded Human liver tissue using anti-Catalase mouse monoclonal antibody.

Flow Cytometry: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Analysis of Jurkat cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.

Immunohistochemistry: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Catalase mouse monoclonal antibody.

Immunohistochemistry: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Catalase mouse monoclonal antibody.

Immunohistochemistry: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Staining of paraffin-embedded Human endometrium tissue using anti-Catalase mouse monoclonal antibody.

Immunohistochemistry: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Staining of paraffin-embedded Human lung tissue using anti-Catalase mouse monoclonal antibody.

Flow Cytometry: Catalase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70342] - Analysis of Hela cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Catalase Antibody (OTI1B8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Catalase Antibody (OTI1B8) - Azide and BSA Free (NBP2-00492) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CAT (NP_111743) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 59.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Catalase Antibody (OTI1B8) - Azide and BSA Free

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAT