Human Caspase-9 ELISA Kit (Colorimetric)

Images

 
ELISA: Caspase-9 ELISA Kit [NBP1-83734] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, cell lysate, serum
Standard Curve Range
1.6 - 100 ng/mL
Sensitivity
0.4 ng/mL

Order Details

Human Caspase-9 ELISA Kit (Colorimetric) Summary

Standard Curve Range
1.6 - 100 ng/mL
Sensitivity
0.4 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
9.0%
Intra-Assay
6.6%
Spike Recovery
The amount of endogenous human Caspase-9 in unspiked lysates was subtracted from the spike values. The overall mean recovery was 103%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CASP9

Applications/Dilutions

Application Notes
The human Caspase-9 ELISA is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-9
  3. Anti-rabbit-IgG-HRP
  4. Anti-human Caspase-9 polyclonal Detection Antibody (rabbit)
  5. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  6. Lysis Buffer 10x
  7. Human Caspase-9 Standard lyophilized, 200 ng/ml upon reconstitution
  8. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  9. Substrate Solution (tetramethyl-benzidine)
  10. Sample Diluent
  11. Stop Solution (1M Phosphoric acid)

Alternate Names for Human Caspase-9 ELISA Kit (Colorimetric)

  • APAF-3APAF3
  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC 3.4.22.62
  • ICE-LAP6CASPASE-9c
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease

Background

Caspases are the executioners of apoptosis. This cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP (3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase-9 thus is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Caspase-9 is essentially localized in mitochondria, the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its subcellular redistribution and activation. Apart from cleavage of caspase-3, the downstream enzymes caspase-6 and 7 are targets of caspase-9 activity. Caspase-9 forms dimers in the course of activation. Cytochrome c/Apaf-1/caspase-9 form a so called apoptosome, amplifying the caspase cascade.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NB100-56116
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56503
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-56074
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-27335
Species: Hu
Applications: WB, Flow, CyTOF-ready
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56106
Species: Hu, Mu, Rt, Ca, Ge
Applications: WB, IHC, IHC-Fr, IHC-P, IP
AF8221
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-43728
Species: Hu
Applications: WB, ICC/IF
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-56311
Species: Hu
Applications: WB, ICC/IF, IHC-P
NBP1-83734
Species: Hu
Applications: ELISA

Publications for Caspase-9 ELISA Kit (NBP1-83734) (0)

There are no publications for Caspase-9 ELISA Kit (NBP1-83734).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Caspase-9 ELISA Kit (NBP1-83734) (0)

There are no reviews for Caspase-9 ELISA Kit (NBP1-83734). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-9 ELISA Kit (NBP1-83734). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Additional Caspase-9 Products

Bioinformatics Tool for Caspase-9 ELISA Kit (NBP1-83734)

Discover related pathways, diseases and genes to Caspase-9 ELISA Kit (NBP1-83734). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Caspase-9 ELISA Kit (NBP1-83734)

Discover more about diseases related to Caspase-9 ELISA Kit (NBP1-83734).
 

Pathways for Caspase-9 ELISA Kit (NBP1-83734)

View related products by pathway.

PTMs for Caspase-9 ELISA Kit (NBP1-83734)

Learn more about PTMs related to Caspase-9 ELISA Kit (NBP1-83734).
 

Research Areas for Caspase-9 ELISA Kit (NBP1-83734)

Find related products by research area.

Blogs on Caspase-9.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Customers Who Bought This Also Bought

Caspase-9 Antibody
NB100-56118

Contact Information

Product PDFs

Review this Product

Be the first to review our Human Caspase-9 ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol CASP9
Entrez