Caspase-9 ELISA Kit


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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, cell lysate, serum

Order Details

Caspase-9 ELISA Kit Summary

Standard Curve Range
1.6 - 100 ng/mL
0.4 ng/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.


Application Notes
The human Caspase-9 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-9.

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-9
  2. Anti-human Caspase-9 polyclonal Detection Antibody (rabbit)
  3. Anti-rabbit-IgG-HRP
  4. Human Caspase-9 Standard lyophilized, 200 ng/ml upon reconstitution
  5. Sample Diluent
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  8. Lysis Buffer 10x
  9. Substrate Solution (tetramethyl-benzidine)
  10. Stop Solution (1M Phosphoric acid)
  11. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for Caspase-9 ELISA Kit

  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • caspase-9
  • EC
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease


Caspases are the executioners of apoptosis. This cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP (3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase-9 thus is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Caspase-9 is essentially localized in mitochondria, the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its subcellular redistribution and activation. Apart from cleavage of caspase-3, the downstream enzymes caspase-6 and 7 are targets of caspase-9 activity. Caspase-9 forms dimers in the course of activation. Cytochrome c/Apaf-1/caspase-9 form a so called apoptosome, amplifying the caspase cascade.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-9 ELISA Kit (NBP1-83734). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

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Blogs on Caspase-9.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CASP9

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