Recombinant Human Caspase 5 Protein

Product Discontinued

Recombinant Human Caspase 5 Protein Summary

• Description: Recombinant protein for Human Caspase 5
  
  Source:E. coli.
  Amino Acid Sequence:(P51878)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab52109)
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CASP5
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
  Specific activity: > 5000 units/mg

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to 1 unit per ul in PBS containing 15% glycerol.

Alternate Names for Recombinant Human Caspase 5 Protein

• CASP5
• CASP-5
• caspase 5, apoptosis-related cysteine peptidase
• caspase 5, apoptosis-related cysteine protease
• Caspase5
• Caspase-5
• EC 3.4.22
• EC 3.4.22.58
• ICE(rel)III
• ICE(rel)-III
• ICEREL-III
• ICH3
• ICH-3
• MGC141966
• Protease ICH-3
• Protease TY
• TY protease

Background

Caspase-5 (also know as ICErelIII, TY) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-5 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-5 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-5 activation and roles of caspase-5 in apoptosis are poorly understood. The recombinant active human caspase-5 was expressed in E. coli. The active caspase-5 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA (Cat. #1102-200) or Ac-WEHD-AFC (1101-200).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP5