Caspase 5 Activity Assay Kit (Colorimetric)

Product Discontinued

Caspase 5 Activity Assay Kit (Colorimetric) Summary

• Description: Simple and convenient colorimetric assay to measure Caspase-5 activity within 1-2 hrs. The kit provides a means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD.
  
  Detection: Absorbance (400 or 405 nm )Applications: Detects specific Caspases involved in apoptosis within cells
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65661)
• Specificity: The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm.
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CASP5

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase 5 Activity Assay Kit (Colorimetric)

• CASP5
• CASP-5
• caspase 5, apoptosis-related cysteine peptidase
• caspase 5, apoptosis-related cysteine protease
• Caspase5
• Caspase-5
• EC 3.4.22
• EC 3.4.22.58
• ICE(rel)III
• ICE(rel)-III
• ICEREL-III
• ICH3
• ICH-3
• MGC141966
• Protease ICH-3
• Protease TY
• TY protease

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-5 (ICH-3, ICEREL-III, TY protease) is a member of the caspase-1 subfamily and it is an upstream mediator of apoptosis. Caspase-5 is highly expressed in lung, liver, and skeletal muscle, and similar to Caspase-4, its overexpression can induce cellular apoptosis (1). A tetramer, Caspase-5 separates into two subunits of a 20kda (p20) and 10kda (p10) after activation. In vitro, Caspase-5 can be cleaved by GranB, a major player in Cytotoxic T lymphocytes (CTL) induced apoptosis (2). In vivo, the Caspase-5 expression is elevated by LPS and IFNg. Caspase-5 induced apoptosis can be inhibited by CrmA and Bcl-2. Caspase-5 has also been implicated in cellular inflammatory response and in the microsatelite mutator pathway for cancer (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP5