Caspase 5 Assay Kit (Fluorometric)

Product Discontinued

Caspase 5 Assay Kit (Fluorometric) Summary

• Description: Simple and convenient fluorometric assay to measure Caspase-5 Activity within 1-2 hrs.
  
  Detection method: Fluorescence (Ex/Em = 400/505 nm)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65660)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CASP5

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase 5 Assay Kit (Fluorometric)

• CASP5
• CASP-5
• caspase 5, apoptosis-related cysteine peptidase
• caspase 5, apoptosis-related cysteine protease
• Caspase5
• Caspase-5
• EC 3.4.22
• EC 3.4.22.58
• ICE(rel)III
• ICE(rel)-III
• ICEREL-III
• ICH3
• ICH-3
• MGC141966
• Protease ICH-3
• Protease TY
• TY protease

Background

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-5 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (WLmax = 400 nm); upon cleavage of the substrate, free AFC emits a yellow-green fluorescence (WLmax = 505 nm), which can be quantified using a fluorometer or fluorescence microplate reader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP5