Carm1 [p Ser228] Antibody - Azide and BSA Free

Western Blot: Carm1 [p Ser228] Antibody [NBP3-16892] - Western blot analysis of extracts of Jurkat cells, using Carm1 antibody (NBP3-16892) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Carm1 [p Ser228] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Carm1 [p Ser228] Antibody - Azide and BSA Free (NBP3-16892) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around S228 of human PRMT4/CARM1 (NP_954592.1). VKSNN
• Modification: p Ser228
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARM1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Carm1 [p Ser228] Antibody - Azide and BSA Free

• Carm1
• coactivator-associated arginine methyltransferase 1EC 2.1.1.-
• EC 2.1.1
• histone-arginine methyltransferase CARM1
• PRMT4
• PRMT4EC 2.1.1.125
• Protein arginine N-methyltransferase 4

Background

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 (PubMed 11724789)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: CARM1