CARD12 Antibody - BSA Free

Western Blot: CARD12 Antibody [NB500-225] - Immunoblot analysis of cN-II and Ipaf (CARD12) protein expression in different human cell lines. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121525) licensed under a CC-BY license.

Western Blot: CARD12 Antibody [NB500-225] - Western Blot analysis of CLAN in lung lysate with anti-CLAN at a 1:500 dilution. Detects CLAN-A and CLAN-B isoforms.

In vitro interaction between cN-II and Ipaf B.After loading Ni-talon beads with E. coli “BL21(DE3)” crude extract without (■) or with cN-II (●), beads were washed and E. coli “BL21 Rosetta(DE3)” extracts containing Ipaf B were loaded. Elution buffer containing 250 mM imidazole was used from fraction 21 to detach His-tagged cN-II and elute proteins. A) proteins elution profile; B) immunoblotting with CARD12 and cN-II antibodies on elution fractions 3, 22, 24, 25 and 26 from chromatography.

Western Blot: CARD12 Antibody - BSA Free [NB500-225] - In vitro interaction between cN-II & Ipaf B.After loading Ni-talon beads with E. coli “BL21(DE3)” crude extract without (■) or with cN-II (●), beads were washed & E. coli “BL21 Rosetta(DE3)” extracts containing Ipaf B were loaded. Elution buffer containing 250 mM imidazole was used from fraction 21 to detach His-tagged cN-II & elute proteins. A) proteins elution profile; B) immunoblotting with CARD12 & cN-II antibodies on elution fractions 3, 22, 24, 25 & 26 from chromatography. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25811392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CARD12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CARD12 Antibody - BSA Free (NB500-225) is a polyclonal antibody validated for use in WB. Anti-CARD12 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: N terminus (MNFIKDNSRALIQRMGM) of the A and B isoforms of the human CLAN protein.
• Localization: Cytoplasmic filaments
• Specificity: Reacts with the N terminus (MNFIKDNSRALIQRMGM) of the A and B isoforms of the human CLAN protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Application Notes: This antibody is useful for western blot analysis where bands are seen around 110 kDa and 40 kDa representing isoforms A and B respectively.

Reactivity Notes

This antibody reacts with human CLAN.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CARD12 Antibody - BSA Free

• CARD12leucine rich repeat and CARD domaincontaining 4
• caspase recruitment domain family, member 12
• Caspase recruitment domain-containing protein 12
• Clan protein
• CLAN1ipaf
• CLANCARD, LRR, and NACHT-containing protein
• CLR2.1
• Ice protease-activating factor
• Ipaf
• NLR family, CARD domain containing 4

Background

A novel human CED-4-like gene, CLAN, is a caspase-associated recruitment domain (CARD)-containing proteins and is expressed at varying degrees in normal human tissues. It contributes to a number of intracellular processes including apoptosis, cytokine processing, and NF-kappa B activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CARD12 Antibody (NB500-225)(1)

Publication using NB500-225

• Cividini F, Tozzi MG, Galli A et al. Cytosolic 5-Nucleotidase II Interacts with the Leucin Rich Repeat of NLR Family Member Ipaf. PLoS One. 2015-03-26 [PMID: 25811392] (WB, Human)

FAQs for CARD12 Antibody (NB500-225). (Showing 1 - 1 of 1 FAQ).

1. What kind of blocking buffer is good for Western blot with CARD12 antibody (NB500-225)? Please tell us % tween in PBS, % milk etc.?
   • For CARD12 antibody NB500-225, we recommend using 5% non-fat dry milk in TBST (0.1% Tween in TBS) for blocking and 1% non-fat dry milk in TBST for primary and secondary diluents. While this is the protocol we like to use, you can amend this buffer to fit your needs. We sometimes add 1% BSA to the blocking buffer for additional blocking if the blot is coming out dirty.

Bioinformatics

• Gene Symbol: NLRC4
• Entrez:
  • 58484(Human)