Carboxylesterase 3/CES3/Esterase 31 Antibody - BSA Free

Western Blot: Carboxylesterase 3/CES3/Esterase 31 Antibody [NBP2-47529] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Analysis of liver tissue

Immunocytochemistry/Immunofluorescence: Carboxylesterase 3/CES3/Esterase 31 Antibody [NBP2-47529] - Staining of human cell line HEK 293 shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Carboxylesterase 3/CES3/Esterase 31 Antibody [NBP2-47529] - Staining of human colon tissue. Shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Carboxylesterase 3/CES3/Esterase 31 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Carboxylesterase 3/CES3/Esterase 31 Antibody - BSA Free (NBP2-47529) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: EGAFVFGGPFLMDESSRLAFPEATEEEKQLSLTMMAQWTHFARTGDPNSKALPPWPQFNQAEQYLEINPVPRAGQKFREAWMQFWSETLPSKIQQWHQKQKNRK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CES3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Carboxylesterase 3/CES3/Esterase 31 Protein (NBP2-47529PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Carboxylesterase 3/CES3/Esterase 31 Antibody - BSA Free

• carboxylesterase 3 (brain)
• Carboxylesterase 3
• CES3
• EC 3.1.1
• EC 3.1.1.1
• ES31FLJ21736
• esterase 31
• Liver carboxylesterase 31 homolog

Background

Carboxylesterase 3 is a member of a large multigene family. The enzymes encoded by these genes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. The specific function of this enzyme has not yet been determined; however, it is speculated that carboxylesterases may play a role in lipid metabolism and/or the blood-brain barrier system. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CES3