Carboxylesterase 1/CES1 Antibody

Western blot shows lysates of mouse liver. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7929) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxylesterase 1/CES1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Carboxylesterase 1/CES1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 1/CES1
  His19-Leu565
  Accession # Q8VCC2
• Specificity: Detects mouse Carboxylesterase 1/CES1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CES3 and recombinant human CES1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Ces1g
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxylesterase 1/CES1 Antibody

• ACAT
• acyl coenzyme A:cholesterol acyltransferase
• Acyl-coenzyme A:cholesterol acyltransferase
• Brain carboxylesterase hBr1
• carboxylesterase 1 (monocyte/macrophage serine esterase 1)
• Carboxylesterase 1
• carboxylesterase 2 (liver)
• CEH
• CES1
• Ces-1
• CES1A2
• CES2EC 3.1.1.1
• cholesteryl ester hydrolase
• Cocaine carboxylesterase
• EC 3.1.1
• Egasyn
• HMSE1
• HMSECES1A1
• human monocyte/macrophage serine esterase 1
• liver carboxylesterase 1
• MGC117365
• Monocyte/macrophage serine esterase
• PCE-1
• REH
• Retinyl ester hydrolase
• Serine esterase 1
• SES1
• Ses-1
• SES1TGH
• TGH
• Triacylglycerol hydrolase

Background

Carboxylesterase 1/CES1 (also known as Ses-1 and Liver carboxylesterase 1) is a 60-63 kDa glycoprotein, member of a type-B carboxylesterase/lipase family localized to microsomal membrane and  lumen of endoplasmic reticulum. CES1 exists as a homotrimer and homohexamer and is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES1 is expressed mostly in liver, and with lower levels found in the lung. Over amino acids (aa) 19‑565, mouse CES1 shares 75% aa identity with human CES1.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ces1g
• Uniprot:
  • Q8VCC2()