Carbonic Anhydrase XIV/CA14 Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Carbonic Anhydrase XIV/CA14 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XIV/CA14
  Ala16-Met290
  Accession # Q9WVT6
• Specificity: Detects mouse Carbonic Anhydrase XIV/CA14 in Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) CA14 is observed and less than 5% cross-reactivity with rhCA1, -2, -3, -6, -8, -10, -13 recombinant mouse CA4, -9, and -12 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Car14
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Carbonic anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Mouse CA14 is a type I membrane enzyme highly expressed in the kidney and heart, followed by the skeletal muscle, brain, lung and liver (2). The deduced amino acid sequence of mouse CA14 consists of a signal peptide (residues 1 to 15), an ectodomain (residues 16 to 290), a transmembrane domain (residues 291 to 311) and a cytoplamic region (residues 32 to 337). rmCA14 corresponds to the ectodomain and has activity as described in Activity Assay Protocol.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. 
2. Mori, K. et al. (1999) J. Biol. Chem. 274:15701.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Car14