VEZT Antibody - BSA Free

Western Blot: VEZT Antibody [NBP2-20855] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VEZT antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: VEZT Antibody [NBP2-20855] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Western Blot: VEZT Antibody [NBP2-20855] - Sample (20 ug of whole cell lysate ) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: VEZT Antibody [NBP2-20855] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:3000.

Western Blot: VEZT Antibody [NBP2-20855] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VEZT antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VEZT Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human VEZT. The exact sequence is proprietary.
• Localization: Cell membrane; Multi-pass membrane protein; Cell junction; adherens junction; Nucleus; Acrosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VEZT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for VEZT Antibody - BSA Free

• DKFZp761C241
• vezatin
• vezatin, adherens junctions transmembrane protein

Background

Plays a pivotal role in the establishment of adherens junctions and their maintenance in adult life. In case of Listeria infection, promotes bacterial internalization by participating to myosin VIIa recruitment to the entry site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: VEZT
• Uniprot:
  • Q9HBM0(Human)