Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] - Samples were spiked with high concentrations of Human Carbonic Anhydrase III/CA3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL

Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CA3 in samples. No significant cross-reactivity or interference between Human CA3 and analogues was observed.
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6.03%
• Intra-Assay: CV% < 5.97%
• Spike Recovery: 87-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CA3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

• CA3
• CAIII
• CA-III
• Car3
• Carbonate dehydratase III
• carbonic anhydrase 3
• Carbonic Anhydrase III
• carbonic anhydrase III, muscle specific
• EC 4.2.1.1

Background

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Carbonic Anhydrase III/CA3 ELISA Kit (NBP2-70057). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CA3