Novus Biologicals products are now on bio-techne.com

Carbonic Anhydrase II/CA2 Antibody [HRP]

Images

 
Dot Blot: Carbonic Anhydrase II/CA2 Antibody [HRP] [NB600-619] - Dot Blot of Rabbit Carbonic Anhydrase II/CA2/CA2 antibody [HRP].Lane 1: 100ng.Lane 2: 33.3ng.Lane 3: 11.1ng.Lane 4: 3.7ng.Lane 5: 1.23ng.Secondary ...read more
ELISA: Carbonic Anhydrase II/CA2 Antibody [HRP] [NB600-619] - ELISA results of purified Carbonic Anhydrase II/CA2/CA2 antibody [HRP] tested against Carbonic Anhydrase II/CA2 (erythrocytes). (Green line) The starting ...read more
Dot Blot of Rabbit Anti-Carbonic Anhydrase II Peroxidase Conjugated Antibody. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: 200-4357 Anti-Carbonic Anhydrase II HRP ...read more

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, DB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Carbonic Anhydrase II/CA2 Antibody [HRP] Summary

Description
This vial contains a relatively low volume of reagent (25 uL). To minimize loss of volume dilute 1:10 by adding 225 uL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing.

Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]
Immunogen
Carbonic Anhydrase II/CA2/CA2 [Bovine Erythrocytes] (Uniprot: P00921)
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CA2
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA 1:5000-1:20000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000-1:5000
Application Notes
This product has been tested by ELISA and dot blot and is assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Readout System
Publications
Read Publication using
NB600-619 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 9336012).

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Purity
Multi-step

Alternate Names for Carbonic Anhydrase II/CA2 Antibody [HRP]

  • CA2
  • CAII
  • CA-IIEC 4.2.1.1
  • Car2
  • Carbonate dehydratase II
  • carbonic anhydrase 2
  • carbonic anhydrase B
  • Carbonic anhydrase C
  • Carbonic Anhydrase II
  • carbonic anhydrase IICAC
  • carbonic dehydratase

Background

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-88191
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00000761-M02
Species: Hu, Mu
Applications: ELISA, Func, IHC, IHC-P, S-ELISA, WB
MAB2186
Species: Hu
Applications: IHC, IP, WB
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF1936
Species: Hu
Applications: IP, WB
DY1707
Species: Hu
Applications: ELISA
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NB300-731
Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
DBD00
Species: Hu
Applications: ELISA
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-49409
Species: Hu
Applications: IHC, IHC-P
NBP1-86616
Species: Hu
Applications: IHC, IHC-P, WB
233-FB
Species: Hu
Applications: BA
NB100-56508
Species: Av, Hu, Mu, Rt
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P, WB
DY805
Species: Hu
Applications: ELISA

Publications for Carbonic Anhydrase II/CA2 Antibody (NB600-619)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Carbonic Anhydrase II/CA2 Antibody (NB600-619) (0)

There are no reviews for Carbonic Anhydrase II/CA2 Antibody (NB600-619). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Carbonic Anhydrase II/CA2 Antibody (NB600-619) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Carbonic Anhydrase II/CA2 Products

Research Areas for Carbonic Anhydrase II/CA2 Antibody (NB600-619)

Find related products by research area.

Blogs on Carbonic Anhydrase II/CA2

There are no specific blogs for Carbonic Anhydrase II/CA2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Carbonic Anhydrase II/CA2 Antibody [HRP] and receive a gift card or discount.

Bioinformatics

Gene Symbol CA2
Uniprot