Carbonic Anhydrase II/CA2 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Carbonic Anhydrase II/CA2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Carbonic Anhydrase II/CA2
  Ser2-Lys260
  Accession # P00918
• Specificity: Detects human Carbonic Anhydrase II/CA2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) CA1, rhCA3 and rhCA13 is observed and less than 5% cross-reactivity with rhCA4, 8, 9, 10, 12, and 14 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase II/CA2 Antibody [Biotin]

• CA2
• CAII
• CA-IIEC 4.2.1.1
• Car2
• Carbonate dehydratase II
• carbonic anhydrase 2
• carbonic anhydrase B
• Carbonic anhydrase C
• Carbonic Anhydrase II
• carbonic anhydrase IICAC
• carbonic dehydratase

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO 2 + H 2 O = HCO 3 - + H + which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6 th International Conference on the CAs, June 20-25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO 2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA2
• Uniprot:
  • P00918()