Carbonic Anhydrase IV/CA4 Antibody (310413) [Unconjugated]

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase IV/CA4 Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
310413
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Carbonic Anhydrase IV/CA4 Antibody (310413) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IV/CA4
Ala19-Lys283
Accession # P22748
Specificity
Detects human Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
10% cross‑reactivity with recombinant human (rh) CA1, rhCA2, and recombinant mouse CA4 is observed. Does not cross-react with rhCA3, 8, 9, 10, 12, 13, or 14.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CA4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
MAB2186 in the following applications:

  • ICC
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IV/CA4 Antibody (310413) [Unconjugated]

  • CA4
  • CAIV
  • CA-IV
  • Car4
  • Carbonate dehydratase IV
  • carbonic anhydrase 4
  • Carbonic Anhydrase IV
  • carbonic anhydrase IVRP17
  • carbonic dehydratase IV
  • EC 4.2.1.1
  • retinitis pigmentosa 17 (autosomal dominant)
  • RP17

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA4 is a
GPI‑anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2 taste sensor (2). In addition, a genetic mutation (Arg 14 to Trp in the signal peptide) of CA4 was found to co‑segregate with the RP17 form of retinitis pigmentosa in two large families and was not found in 36 unaffected family members or 100 controls (3).

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Chandrashekar, J. et al. (2009) Science 326:443.
  3. Rebello, G. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6617.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase IV/CA4 Antibody (MAB2186)(3)

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Bioinformatics

Gene Symbol CA4
Entrez
Uniprot