CaMKIV Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CaMKIV Antibody [NBP1-88210] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.

Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.

Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neurons.

Staining of human skin shows no positivity in squamous epithelial cells as expected.

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CaMKIV Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CaMKIV Antibody - BSA Free (NBP1-88210) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GNEDMKAIPEGEKIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAMK4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CaMKIV Protein (NBP1-88210PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CaMKIV Antibody - BSA Free

• brain Ca(2+)-calmodulin-dependent protein kinase type IV
• brain Ca++-calmodulin-dependent protein kinase type IV
• calcium/calmodulin-dependent protein kinase IV
• calcium/calmodulin-dependent protein kinase type IV catalytic chain
• calcium/calmodulin-dependent protein kinase type IV
• CAM kinase- GR
• CAM kinase IV
• CaM kinase-GR
• CaMK IV
• caMK
• CaMK-GR
• EC 2.7.11
• EC 2.7.11.17
• IV
• MGC36771

Background

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK4