Calretinin Antibody [Unconjugated]


Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) more
Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC

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Calretinin Antibody [Unconjugated] Summary

E. coli-derived recombinant human Calretinin
Accession # AAH15484
Detects human, mouse, and rat Calretinin in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF5065 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calretinin Antibody [Unconjugated]

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR


Calretinin (also 29 kDa Calbindin and Calbindin 2) is a 30 kDa member of the EF-hand family of Ca2+-binding proteins. It serves as both an intracellular calcium sensor and calcium buffer. Calretinin is found in cerebellar granule cells plus cortical bipolar and multipolar GABAergic interneurons.

Human Calretinin is 271 amino acids (aa) in length. It contains six EF-hand domains (aa 16 - 270), each of which show a 12 aa Ca2+-binding motif. There are multiple splice variants. One shows a seven aa substitution for aa 181 - 190, accompanied by a 25 aa substitution for the C-terminal Met. Two others show a one and 14 aa substitution for aa 179 - 271, generating a 20 kDa and 22 kDa isoform, respectively. A fourth variant contains a seven aa substitution for aa 185 - 271. Over aa 2 - 177, human and mouse Calretinin show complete aa identity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calretinin Antibody (AF5065)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IHC, IHC-P.

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Showing Publications 1 - 6 of 6.
Publications using AF5065 Applications Species
CE Geisler, S Ghimire, C Hepler, KE Miller, SM Bruggink, KP Kentch, MR Higgins, CT Banek, J Yoshino, S Klein, BJ Renquist Hepatocyte membrane potential regulates serum insulin and insulin sensitivity by altering hepatic GABA release Cell Reports, 2021;35(13):109298. 2021-01-01 [PMID: 34192533] (IHC, Mouse) IHC Mouse
H Beard, G Chidlow, D Neumann, N Nazri, M Douglass, PJ Trim, MF Snel, RJ Casson, KM Hemsley Is the eye a window to the brain in Sanfilippo syndrome? Acta Neuropathol Commun, 2020;8(1):194. 2020-01-01 [PMID: 33203474] (IHC, Mouse) IHC Mouse
MM Hossain, T Tsuzuki, K Sakakibara, F Imaizumi, A Ikegaya, M Inagaki, I Takahashi, T Ito, H Takamatsu, A Kumanogoh, T Negishi, K Yukawa PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice PLoS ONE, 2019;14(8):e0221440. 2019-01-01 [PMID: 31430342] (IHC, Mouse) IHC Mouse
S Panda, P Dohare, S Jain, N Parikh, P Singla, R Mehdizadeh, DW Klebe, GM Kleinman, B Cheng, P Ballabh Estrogen treatment reverses prematurity-induced disruption in cortical interneuron population J. Neurosci., 2018;0(0):. 2018-01-01 [PMID: 30037831] (IHC, Mouse) IHC Mouse
RW Nickells, HM Schmitt, ME Maes, CL Schlamp AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury Invest. Ophthalmol. Vis. Sci., 2017;58(14):6091-6104. 2017-01-01 [PMID: 29204649] (IHC-P, Mouse) IHC-P Mouse
V Kafetzopou, N Kokras, I Sotiropoul, JF Oliveira, H Leite-Alme, A Vasalou, VM Sardinha, Z Papadopoul, OF Almeida, K Antoniou, N Sousa, C Dalla The nucleus reuniens: a key node in the neurocircuitry of stress and depression Mol. Psychiatry, 2017;0(0):. 2017-01-01 [PMID: 28397837] (IHC, Rat) IHC Rat

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Gene Symbol CALB2