Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) ...read more
Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Calretinin Antibody [Unconjugated]
29 kDa calbindin
calbindin 2, (29kD, calretinin)
calbindin 2, 29kDa (calretinin)
Calretinin (also 29 kDa Calbindin and Calbindin 2) is a 30 kDa member of the EF-hand family of Ca2+-binding proteins. It serves as both an intracellular calcium sensor and calcium buffer. Calretinin is found in cerebellar granule cells plus cortical bipolar and multipolar GABAergic interneurons.
Human Calretinin is 271 amino acids (aa) in length. It contains six EF-hand domains (aa 16 - 270), each of which show a 12 aa Ca2+-binding motif. There are multiple splice variants. One shows a seven aa substitution for aa 181 - 190, accompanied by a 25 aa substitution for the C-terminal Met. Two others show a one and 14 aa substitution for aa 179 - 271, generating a 20 kDa and 22 kDa isoform, respectively. A fourth variant contains a seven aa substitution for aa 185 - 271. Over aa 2 - 177, human and mouse Calretinin show complete aa identity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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