Calretinin Antibody [Unconjugated]

Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calretinin at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Calretinin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Calretinin
  Ala2-Ser177
  Accession # AAH15484
• Specificity: Detects human, mouse, and rat Calretinin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CALB2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calretinin Antibody [Unconjugated]

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Background

Calretinin (also 29 kDa Calbindin and Calbindin 2) is a 30 kDa member of the EF-hand family of Ca²⁺-binding proteins. It serves as both an intracellular calcium sensor and calcium buffer. Calretinin is found in cerebellar granule cells plus cortical bipolar and multipolar GABAergic interneurons.

Human Calretinin is 271 amino acids (aa) in length. It contains six EF-hand domains (aa 16 - 270), each of which show a 12 aa Ca²⁺-binding motif. There are multiple splice variants. One shows a seven aa substitution for aa 181 - 190, accompanied by a 25 aa substitution for the C-terminal Met. Two others show a one and 14 aa substitution for aa 179 - 271, generating a 20 kDa and 22 kDa isoform, respectively. A fourth variant contains a seven aa substitution for aa 185 - 271. Over aa 2 - 177, human and mouse Calretinin show complete aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calretinin Antibody (AF5065)(6)

Publications using AF5065

• CE Geisler, S Ghimire, C Hepler, KE Miller, SM Bruggink, KP Kentch, MR Higgins, CT Banek, J Yoshino, S Klein, BJ Renquist Hepatocyte membrane potential regulates serum insulin and insulin sensitivity by altering hepatic GABA release Cell Reports, 2021;35(13):109298. 2021-01-01 [PMID: 34192533] (IHC, Mouse)
• H Beard, G Chidlow, D Neumann, N Nazri, M Douglass, PJ Trim, MF Snel, RJ Casson, KM Hemsley Is the eye a window to the brain in Sanfilippo syndrome? Acta Neuropathol Commun, 2020;8(1):194. 2020-01-01 [PMID: 33203474] (IHC, Mouse)
• MM Hossain, T Tsuzuki, K Sakakibara, F Imaizumi, A Ikegaya, M Inagaki, I Takahashi, T Ito, H Takamatsu, A Kumanogoh, T Negishi, K Yukawa PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice PLoS ONE, 2019;14(8):e0221440. 2019-01-01 [PMID: 31430342] (IHC, Mouse)
• S Panda, P Dohare, S Jain, N Parikh, P Singla, R Mehdizadeh, DW Klebe, GM Kleinman, B Cheng, P Ballabh Estrogen treatment reverses prematurity-induced disruption in cortical interneuron population J. Neurosci., 2018;0(0):. 2018-01-01 [PMID: 30037831] (IHC, Mouse)
• RW Nickells, HM Schmitt, ME Maes, CL Schlamp AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury Invest. Ophthalmol. Vis. Sci., 2017;58(14):6091-6104. 2017-01-01 [PMID: 29204649] (IHC-P, Mouse)
• V Kafetzopou, N Kokras, I Sotiropoul, JF Oliveira, H Leite-Alme, A Vasalou, VM Sardinha, Z Papadopoul, OF Almeida, K Antoniou, N Sousa, C Dalla The nucleus reuniens: a key node in the neurocircuitry of stress and depression Mol. Psychiatry, 2017;0(0):. 2017-01-01 [PMID: 28397837] (IHC, Rat)

Bioinformatics

• Gene Symbol: CALB2
• Uniprot:
  • AAH15484()