Calretinin Antibody

Images

 
Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Calretinin Antibody Summary

Immunogen
E. coli-derived recombinant human Calretinin
Ala2-Ser177
Accession # AAH15484
Specificity
Detects human, mouse, and rat Calretinin in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 5% cross‑reactivity with recombinant human Calbindin D.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CALB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF5065 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calretinin Antibody

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR

Background

Calretinin (also 29 kDa Calbindin and Calbindin 2) is a 30 kDa member of the EF-hand family of Ca2+-binding proteins. It serves as both an intracellular calcium sensor and calcium buffer. Calretinin is found in cerebellar granule cells plus cortical bipolar and multipolar GABAergic interneurons.

Human Calretinin is 271 amino acids (aa) in length. It contains six EF-hand domains (aa 16 - 270), each of which show a 12 aa Ca2+-binding motif. There are multiple splice variants. One shows a seven aa substitution for aa 181 - 190, accompanied by a 25 aa substitution for the C-terminal Met. Two others show a one and 14 aa substitution for aa 179 - 271, generating a 20 kDa and 22 kDa isoform, respectively. A fourth variant contains a seven aa substitution for aa 185 - 271. Over aa 2 - 177, human and mouse Calretinin show complete aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003347-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
AF1107
Species: Mu
Applications: WB, IHC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NBP2-50028
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P, IHC-FrFl
NB120-11427
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-69571
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-15895
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB600-534
Species: Hu, Mu, Po, V-Vi
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-87581
Species: Hu
Applications: WB, IHC, IHC-P
AF3846
Species: Hu, Mu, Rt
Applications: WB, IHC
NB100-56888
Species: Hu, Mu, Rt, Ca, Eq
Applications: WB
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-67691
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB300-560
Species: Hu, Mu, Rt, Av, Bv, Ma, Pm
Applications: WB, IHC, IHC-Fr, IHC-P, IF
AF5065
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for Calretinin Antibody (AF5065)(4)

Reviews for Calretinin Antibody (AF5065) (0)

There are no reviews for Calretinin Antibody (AF5065). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Calretinin Antibody (AF5065) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Calretinin Products

Bioinformatics Tool for Calretinin Antibody (AF5065)

Discover related pathways, diseases and genes to Calretinin Antibody (AF5065). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Calretinin Antibody (AF5065)

Discover more about diseases related to Calretinin Antibody (AF5065).
 

Pathways for Calretinin Antibody (AF5065)

View related products by pathway.

PTMs for Calretinin Antibody (AF5065)

Learn more about PTMs related to Calretinin Antibody (AF5065).

Blogs on Calretinin.

Using RPE65 as a tool to investigate ocular gene therapies
While not life threatening, blindness and retinal disease are profoundly debilitating and greatly affect quality of life.  Understandably, gene therapy has been subject to controversy given it’s potential effects on the rest of our cellular...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Calretinin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CALB2
Uniprot