Calretinin Antibody (rCALB2/7123)

Formalin-fixed, paraffin-embedded human brain stained with Calretinin antibody (rCALB2/7123). Inset: PBS instead of primary, secondary negative control.

Western Blot Analysis of Human Brain tissue lysate using Calretinin Antibody (rCALB2/7123).

• Reactivity: Hu
• Applications: IHC
• Clone: rCALB2/7123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Calretinin Antibody (rCALB2/7123) Summary

• Description: Positive Controls: Human brain, testis or mesothelioma tissue.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human Calretinin protein fragment (around aa23-242) (exact sequence is proprietary)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Calretinin Antibody (rCALB2/7123)

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALB2