Calretinin Antibody (CL12836) - Azide and BSA Free

Analysis in human cerebral cortex and liver tissues using NBP3-44169 antibody. Corresponding Calretinin RNA-seq data are presented for the same tissues.

Staining of human cerebellum shows moderate to strong positivity in granular layer cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human ovarian cancer (granular cell carcinoma) shows moderate cytoplasmic positivity in tumor cells.

Staining of mouse cerebellum shows strong cytoplasmic positivity in cells in granular layer.

Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.

Analysis in human cell line U-2 OS and human cell line SK-MEL-30.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: CL12836
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Calretinin Antibody (CL12836) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Calretinin Antibody (CL12836) - Azide and BSA Free (NBP3-18575) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P22676, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Calretinin Antibody (CL12836) - Azide and BSA Free

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALB2