Calpain 1 Antibody - BSA Free

Staining of human cell line A-431 shows localization to cytosol.

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205] - Staining in human fallopian tube and testis tissues using anti-CAPN1 antibody. Corresponding CAPN1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205] - Staining of human fallopian tube shows high expression.

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205] - Staining of human testis shows low expression as expected.

Analysis in human cell line A-431.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calpain 1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:250
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 30154452). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Calpain 1 Protein (NBP1-88205PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%), Mouse reactivity reported in the scientific literature (PMID: 30154452)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Calpain 1 Antibody - BSA Free

• Calcium-activated neutral proteinase 1
• Calpain 1
• calpain 1, (mu/I) large subunit
• Calpain mu-type
• calpain, large polypeptide L1
• calpain-1 catalytic subunit
• Calpain-1 large subunit
• CANP 1
• CANP
• CANPL1
• CANPL1EC 3.4.22.52
• CAPN1
• Cell proliferation-inducing gene 30 protein
• cell proliferation-inducing protein 30
• EC 3.4.22
• EC 3.4.22.52
• Micromolar-calpain
• muCANP
• muCANPCANP1
• muCL
• PIG30

Background

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calpain 1 Antibody (NBP1-88205)(1)

Publication using NBP1-88205

• Jiang Z, Bo L, et al. Overexpression of homeodomain-interacting protein kinase 2 (HIPK2) attenuates sepsis-mediated liver injury by restoring autophagy. Cell Death Dis 2018-08-28 [PMID: 30154452] (WB, Mouse)

Bioinformatics

• Gene Symbol: CAPN1
• Uniprot:
  • P07384(Human)