Calnexin Antibody - BSA Free

Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485] - Analysis of human spleen tissue stained with Calnexin, pAb at 10ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, Dr, Gp, Ha, Pm, Rb, Sh, Xp
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, MS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calnexin Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of canine calnexin.
• Marker: Endoplasmic Reticulum Membrane Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CANX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10-1:500
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:2000
  • Western Blot 1:1000
• Application Notes: Detects a band of ~90kDa by Western blot. Flow Cytometry was reported in scientific literature.

Reactivity Notes

Quail (100%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Calnexin Antibody - BSA Free

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calnexin Antibody (NBP1-97485)(12)

Publications using NBP1-97485

• GOmez-Puerta S, Ferrero R, Hochstoeger T et al. Live imaging of the co-translational recruitment of XBP1 mRNA to the ER and its processing by diffuse, non-polarized IRE1 alpha eLife 2022-06-22 [PMID: 35730412] (WB)
• Santamaria M, Pardo-Saganta A, Alvarez-Asiain L et al. Nuclear alpha1-Antichymotrypsin Promotes Chromatin Condensation and Inhibits Proliferation of Human Hepatocellular Carcinoma Cells. Gastroenterology 2013-01-04 [PMID: 23295442] (WB, Human)
• L Scorrano et al. Mitofusin 2 tethers endoplasmic reticulum to mitochondria. Nature 456, 605 . 2008-01-01 [PMID: 19052620] (ICC/IF, FLOW, Human)
• J Oberwinkler et al. Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic beta cells. Nat. Cell Biol. 10, 1421 . 2008-01-01 [PMID: 18978782] (WB, Human)
• M Knepper et al. LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat. Am. J. Physiol. Renal Physiol. 295, F1799 . 2008-01-01 [PMID: 18922883] (WB, Rat)
• G Barton et al. The ectodomain of Toll-like recepto r9 is cleaved to generate a functional receptor. Nature 456, 658 . 2008-01-01 [PMID: 18820679] (WB, Mouse)
• RS Hegde et al. Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane. J. Cell Biol. 160, 529 . 2003-01-01 [PMID: 12578908] (WB, Porcine, Canine)
• LJ Parent et al. Specificity of plasma membrane targeting by the rous sarcoma virus gag protein. J. Virol. 77, 470 . 2003-01-01 [PMID: 12477852] (ICC/IF)
• K Nagata et al. Type XXVI collagen, a new member of the collagen family, is specifically expressed in the testis and ovary. J. Biol. Chem. 277, 37678 . 2002-01-01 [PMID: 12145293] (ICC/IF, Primate)
• M Edidin et al. Probing for membrane domains in the endoplasmic reticulum: retention and degradation of unassembled MHC class I molecules. Mol. Biol. Cell 13, 1566 . 2002-01-01 [PMID: 12006653] (IP, Mouse)
• SW Cushman et al. The export of major histocompatibility complex class i molecules from the endoplasmic reticulum of rat brown adipose cells is acutely stimulated by insulin. Mol. Biol. Cell 12, 101. 2001-01-01 [PMID: 11160826] (ICC/IF, Rat)
• KP Kearse et al. T cell receptor assembly and expression in the absence of calnexin. Arch. Biochem. Biophys. 378, 182 . 2000-01-01 [PMID: 10871059] (IP, Human)

Bioinformatics

• Gene Symbol: CANX
• Entrez:
  • 403908(Canine)
  • 821(Human)
  • 12330(Mouse)
  • 29144(Rat)
• Uniprot:
  • P24643(Canine)