Calmodulin 3 Antibody - BSA Free

Immunohistochemistry: Calmodulin 3 Antibody [NBP3-35441] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Calmodulin 3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Western Blot: Calmodulin 3 Antibody [NBP3-35441] - Western blot analysis of various lysates using Calmodulin 3 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calmodulin 3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Calmodulin 3 Antibody - BSA Free (NBP3-35441) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-140 of human Calmodulin 3 (NP_005175.2).
  
  Sequence:
  TDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CALM3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:100 - 1:500
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Calmodulin 3 Antibody - BSA Free

• CALM
• CALM1
• CALM2
• CALML2
• calmodulin 3 (phosphorylase kinase, delta)
• calmodulin
• CAM
• CAM1
• CAM2
• CAM3
• CAMB
• CAMC
• CAMIII
• PHKD
• PHKD3

Background

Calmodulin 3 belongs to the calmodulin family and is involved in mediating the control of several enzymes, protein kinases, ion channels and phosphatases by Ca(2+). This protein is known to have interactions with CACNA1C, KCNH1, FKBP8, RALA, and RALB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALM3