CALML3 Antibody - Azide and BSA Free

Western Blot: CALML3 Antibody [NBP3-00001] - Anti-CALML3 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDa

Immunohistochemistry-Paraffin: CALML3 Antibody [NBP3-00001] - Immunochemical staining of human CALML3 in human breast carcinoma (from 4 donors) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Immunoprecipitation: CALML3 Antibody [NBP3-00001] - CALML3 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate 2 ul anti-CALML3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CALML3 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CALML3 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human CALML3 (Accession#: P27482; Met1-Lys149)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CALML3
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 4-6 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CALML3 Antibody - Azide and BSA Free

• calmodulin-like 3
• calmodulin-like protein 3
• CaM-like protein
• CLPCalmodulin-related protein NB-1

Background

CALML3 may be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, withdifferent affinities, to cellular substrates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALML3