Calcitonin Antibody (PD00-46)

Immunohistochemistry: Calcitonin Antibody (PD00-46) [NBP3-32101] - Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Rabbit anti-Calcitonin antibody (NBP3-32101) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32101) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu
• Applications: IHC
• Clone: PD00-46
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Calcitonin Antibody (PD00-46) Summary

• Description: Novus Biologicals Rabbit Calcitonin Antibody (PD00-46) (NBP3-32101) is a recombinant monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human Calcitonin aa 85-116. (Uniprot: P01258)
• Localization: Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CALCA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000-1:5000
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Calcitonin Antibody (PD00-46)

• ATP2B2plasma membrane calcium ATPase
• ATPase, Ca++ transporting, plasma membrane 4
• CALC1
• CALCA
• Calcitonin
• DKFZp686G08106
• DKFZp686M088
• EC 3.6.3
• EC 3.6.3.8
• Katacalcin
• Matrix-remodeling-associated protein 1
• matrix-remodelling associated 1
• MXRA1
• PCT
• Plasma membrane calcium ATPase isoform 4
• Plasma membrane calcium pump isoform 4
• plasma membrane calcium pump
• plasma membrane calcium-transporting ATPase 4
• PMCA4b
• PMCA4PMCA4x
• sarcolemmal calcium pump

Background

Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALCA