Cadherin-17 Antibody (1262C) [Unconjugated]

Western blot shows lysates of mouse colon tissue and mouse spleen tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Cadherin-17 Monoclonal Antibody (Catalog # MAB8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin-17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cadherin-17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin-17 Monoclonal Antibody (Catalog # MAB8524) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑17 at approximately 153 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC
• Clone: 1262C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cadherin-17 Antibody (1262C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-17
  Glu22-Met786
  Accession # Q9R100
• Specificity: Detects mouse Cadherin-17 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Cdh17
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-17 Antibody (1262C) [Unconjugated]

• cadherin 17, LI cadherin (liver-intestine)
• cadherin
• cadherin-16
• Cadherin17
• Cadherin-17
• CDH16
• CDH17
• FLJ26931
• HPT-1 cadherin
• HPT1
• HPT-1
• human intestinal peptide-associated transporter HPT-1
• human peptide transporter 1
• Intestinal peptide-associated transporter HPT-1
• LI cadherin
• LI-cadherin
• Liver-intestine cadherin
• MGC138218
• MGC142024

Background

Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-17 Antibody (MAB8524)(1)

Publication using MAB8524

• Gonzalez RS, Cates JMM, Revetta F et al. Gastric Carcinomas With Lymphoid Stroma: Categorization and Comparison With Solid-Type Colonic Carcinomas Am. J. Clin. Pathol. 2017 Nov 20 [PMID: 29126141] (IHC, Human)

Bioinformatics

• Gene Symbol: Cdh17
• Uniprot:
  • Q9R100()