Cadherin-11 Antibody (3C8) - Azide and BSA Free

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection against CHD11-His tag (61.49 KDa).

Immunocytochemistry/Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml]

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Analysis of CDH11 expression in MCF-7.

Western Blot: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Analysis of CDH11 expression in human kidney.

Immunocytochemistry/Immunofluorescence: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).

ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.

ELISA: Cadherin-11 Antibody (3C8) [H00001009-M05-100ug] - Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cadherin-11 Antibody (3C8) - Azide and BSA Free Summary

• Immunogen: CDH11 (P55287, 54 a.a. ~ 612 a.a) partial recombinant protein with Fc tag at C terminal. MW of the Fc tag alone is 50 KDa. GWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHETYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVMITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKKPVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTVTVKIAVEDADEPPMFLAPSYIHEVQENAAAGTVVGRVHAKDPDAANSPIRYSIDRHTDLDRFFTINPEDGFIKTTKPLDREETAWLNITVFAAEIHNRHQEAKVPVAIRVLDVNDNAPKFAAPYEGFICESDQTKPLSNQPIVTISADDKDDTANGPRFIFSLPPEIIHNPNFTVRDNRDNTAGVYARRGGFSRQKQDLYLLPIVISDGGIPPMSSTNTLTIKVCGCDVNGALLSCNAEAYILN
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH11
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cadherin-11 Antibody (3C8) - Azide and BSA Free

• CAD11OSF-4
• cadherin 11, type 2, OB-cadherin (osteoblast)
• Cadherin11
• Cadherin-11
• CDH11
• CDHOB
• OB
• OB-Cadherin
• Osteoblast cadherin

Background

Cadherin-11 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. Cadherin-11 is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH11
• Entrez:
  • 1009(Human)
• OMIM:
  • 600023(Human)
• Uniprot:
  • P55287(Human)