C9orf72 Antibody (779) - Azide and BSA Free

Western Blot: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 Antibody (GT779) [NBP3-13507] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: C9orf72 Antibody (GT779) [NBP3-13507] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Western Blot: C9orf72 Antibody (GT779) [NBP3-13507] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: C9orf72 Antibody (GT779) [NBP3-13507] - Human colon cancer. C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 Antibody (GT779) [NBP3-13507] - Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 ug of C9orf72 antibody [779] (NBP3-13507). Western blot analysis was performed using C9orf72 antibody [779] (NBP3-13507). EasyBlot anti-Mouse IgG was used as a secondary reagent.

Immunocytochemistry/ Immunofluorescence: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 stained by C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.Blue: Fluoroshield with DAPI .

Western Blot: C9orf72 Antibody (779) [NBP3-13507] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clone: 779
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

C9orf72 Antibody (779) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.
• Predicted Species: Xenopus (90%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: C9orf72
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for C9orf72 Antibody (779) - Azide and BSA Free

• ALSFTD
• C9orf72
• chromosome 9 open reading frame 72
• DENNL72
• FTDALS
• FTDALS1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C9orf72