C1qTNF3/CORS26/CTRP3 Antibody [Unconjugated]

Western blot shows lysates of human mesenchymal stem cells either undifferentiated or differentiated into adipocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CTRP3/C1qTNF3/CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTRP3/C1qTNF3/CORS26 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CTRP3/C1qTNF3/CORS26 was detected in immersion fixed human mesenchymal stem cells differentiated (upper panel) or not (lower panel) into adipocytes using Sheep Anti-Human CTRP3/C1qTNF3/CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

C1qTNF3/CORS26/CTRP3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CTRP3/C1qTNF3/CORS26
  Asp24-Lys246
  Accession # Q9BXJ4
• Specificity: Detects human CTRP3/C1qTNF3/CORS26 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse CTRP3/C1qTNF3/CORS26 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: C1QTNF3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C1qTNF3/CORS26/CTRP3 Antibody [Unconjugated]

• C1ATNF3
• C1q and tumor necrosis factor related protein 3
• C1qTNF3
• Cartducin
• cartonectin
• collagenous repeat-containing sequence of 26-kDa
• complement C1q tumor necrosis factor-related protein 3
• complement-c1q tumor necrosis factor-related protein 3
• Corcs
• Cors
• CORS26
• Cors-26
• CTRP3
• CTRP32310005P21Rik
• FLJ37576
• Secretory protein CORS26

Background

C1qTNF3 (Complement C1q TNF-related protein 3/CTRP3; also CORS26 and cartonectin) is a 30-32 kDa, secreted member of the C1q and TNF-related protein (CTRP) superfamily of molecules. It is expressed by a wide variety of cells, including smooth muscle cells, fibroblasts, adipocytes, monocytes and proliferating chondrocytes. C1qTNF3 is an anti-inflammatory agent that apparently blocks LPS activation of mononuclear cells. It also has marked proliferative activity on diverse cell types such as vascular smooth muscle, chrondrocytes, and endothelium. Finally, C1qTNF3 is known to act on hepatocytes and suppress hepatocyte gluconeogenesis. Mature human C1qTNF3 is 224 amino acids (aa) in length (aa 23-246). It possesses an N-terminal collagen-like domain (aa 51-113) followed by a
C-terminal globular region (aa 113-246). C1qTNF3 is monomeric when intracellular, but forms a 90 kDa homotrimer plus higher-order oligomer when secreted. There are at least two potential isoform variants. One is 40-42 kDa, glycosylated, and contains a 73 aa insertion after Glu28, while a second shows concurrent deletions of
aa 46‑69 and 82-105. The longer 40 kDa isoform is reported to form heterotrimers and oligomers with the standard 30 kDa isoform. This has the effect of protecting the standard isoform from proteolysis. Over aa 24-246, human C1qTNF3 shares 99% aa sequence identity with mouse C1qTNF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C1qTNF3/CORS26/CTRP3 Antibody (AF7925)(1)

Publication using AF7925

• Ying Li, Gary L. Wright, Jonathan M. Peterson C1q/TNF‐Related Protein 3 (CTRP3) Function and Regulation Comprehensive Physiology 2017-06-23 [PMID: 28640446]

Bioinformatics

• Gene Symbol: C1QTNF3
• Uniprot:
  • Q9BXJ4()