Recombinant Mouse C-Reactive Protein/CRP Protein

Product Discontinued

Recombinant Mouse C-Reactive Protein/CRP Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 20-225 of Mouse C-Reactive Protein/CRP
  
  

  Source: E.coli

  Amino Acid Sequence: HEDMFKKAFV FPKESDTSYV SLEAESKKPL NTFTVCLHFY TALSTVRSFS VFSYATKKNS NDILIFWNKD KQYTFGVGGA EVRFMVSEIP EAPTHICASW ESATGIVEFW IDGKPKVRKS LHKGYTVGPD ASIILGQEQD SYGGDFDAKQ SLVGDIGDVN MWDFVLSPEQ ISTVYVGGTL SPNVLNWRAL NYKAQGDVFI KPQLWSLEHH HHHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CRP
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 24.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 0.1 M PMSF, 40% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse C-Reactive Protein/CRP Protein

• C-Reactive Protein
• C-reactive protein, pentraxin-related
• CRP
• MGC88244
• pentraxin 1
• PTX1MGC149895

Background

C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CRP