Western blot shows lysate of mouse serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1829) followed by HRP-conjugated ...read more
Simple Western lane view shows mouse serum and mouse plasma, loaded at 0.2 mg/mL. A specific band was detected for C‑Reactive Protein/CRP at approximately 30 kDa (as indicated) using 10 µg/mL of Goat ...read more
C‑Reactive Protein/CRP was detected in perfusion fixed paraffin-embedded sections of Mouse Liver using Goat Anti-Mouse C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for C-Reactive Protein/CRP Antibody [Unconjugated]
C-reactive protein, pentraxin-related
C-reactive protein (CRP) is a member of the pentraxin family of plasma proteins that are part of the lectin fold superfamily of calcium-dependent, carbohydrate-binding proteins (1). CRP is named for its ability to bind to the C-polysaccharide of Strep. Pneumoniae. CRP is characterized by cyclic pentameric structure that contains five identical protomers/subunits, each exhibiting a lectin fold composed of two antiparallel beta -sheets with a fattened jellyroll topology. The mouse CRP precursor is 225 amino acids (aa) in length and contains a signal peptide of 19 aa with a mature polypeptide of 206 aa (2, 3). There is one intrachain disulfide bond and no N-linked glycosylation site(s). Although rat CRP is glycosylated at an N-linked site, human, mouse and rabbit CRP all appear to be non-glycosylated (1, 4, 5). In mouse, the protomers are assembled non-covalently to form the pentameter; in rat, two of the five protomers are covalently linked (6). Mature mouse CRP shares 74%, 71%, 79%, and 68% aa sequence identity with rat, human, hamster and guinea pig CRP, respectively. In human, CRP is induced in hepatocytes principally by IL-6 (1). In mouse, IL-6 has very little effect. Mouse CRP induction is due principally to IL-1 (1, 7), with another pentraxin, SAP, being IL-6 inducible (7). CRP exhibits calcium-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal cell walls, is a principal ligand of CRP. CRP will also bind to the cell membrane of injured necrotic and apoptotic cells. In this context, CRP acts as an opsonin, binding to Fc gamma RI and II, and serves as an antiinflammatory agent (8).
Hirschfield, G.M. and M.B. Pepys (2003) Q.J. Med 96:793.
Whitehead, A.S. et al. (1990) Biochem. J. 266:283.
Ohnishi, S. et al. (1988) Biochem. Biophys. Res. Commun. 156:814.
Sambasivam, H. et al. (1993) J. Biol. Chem. 268:10007.
Black, S. et al. (2004) J. Biol. Chem. 279:48487.
Rassouli, M. et al. (1992) J. Biol. Chem. 267:2947.
Ku, N.-O. and R.F. Mortensen (1993) Cytokine 5:319.
Mold, C. et al. (2002) J. Immunol. 169:7019.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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