C-Reactive Protein/CRP Antibody [Unconjugated]

Images

 
Western blot shows lysate of mouse serum. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1829) followed by HRP-conjugated ...read more
Simple Western lane view shows mouse serum and mouse plasma, loaded at 0.2 mg/mL. A specific band was detected for C‑Reactive Protein/CRP at approximately 30 kDa (as indicated) using 10 µg/mL of Goat ...read more
C‑Reactive Protein/CRP was detected in perfusion fixed paraffin-embedded sections of Mouse Liver using Goat Anti-Mouse C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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C-Reactive Protein/CRP Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse C‑Reactive Protein/CRP
His20-Ser225
Accession # Q91XB3
Specificity
Detects mouse C‑Reactive Protein/CRP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1829 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C-Reactive Protein/CRP Antibody [Unconjugated]

  • C-Reactive Protein
  • C-reactive protein, pentraxin-related
  • CRP
  • MGC88244
  • pentraxin 1
  • PTX1MGC149895

Background

C-reactive protein (CRP) is a member of the pentraxin family of plasma proteins that are part of the lectin fold superfamily of calcium-dependent, carbohydrate-binding proteins (1). CRP is named for its ability to bind to the C-polysaccharide of Strep. Pneumoniae. CRP is characterized by cyclic pentameric structure that contains five identical protomers/subunits, each exhibiting a lectin fold composed of two antiparallel beta -sheets with a fattened jellyroll topology. The mouse CRP precursor is 225 amino acids (aa) in length and contains a signal peptide of 19 aa with a mature polypeptide of 206 aa (2, 3). There is one intrachain disulfide bond and no N-linked glycosylation site(s). Although rat CRP is glycosylated at an N-linked site, human, mouse and rabbit CRP all appear to be non-glycosylated (1, 4, 5). In mouse, the protomers are assembled non-covalently to form the pentameter; in rat, two of the five protomers are covalently linked (6). Mature mouse CRP shares 74%, 71%, 79%, and 68% aa sequence identity with rat, human, hamster and guinea pig CRP, respectively. In human, CRP is induced in hepatocytes principally by IL-6 (1). In mouse, IL-6 has very little effect. Mouse CRP induction is due principally to IL-1 (1, 7), with another pentraxin, SAP, being IL-6 inducible (7). CRP exhibits calcium-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal cell walls, is a principal ligand of CRP. CRP will also bind to the cell membrane of injured necrotic and apoptotic cells. In this context, CRP acts as an opsonin, binding to Fc gamma RI and II, and serves as an antiinflammatory agent (8).

  1. Hirschfield, G.M. and M.B. Pepys (2003) Q.J. Med 96:793.
  2. Whitehead, A.S. et al. (1990) Biochem. J. 266:283.
  3. Ohnishi, S. et al. (1988) Biochem. Biophys. Res. Commun. 156:814.
  4. Sambasivam, H. et al. (1993) J. Biol. Chem. 268:10007.
  5. Black, S. et al. (2004) J. Biol. Chem. 279:48487.
  6. Rassouli, M. et al. (1992) J. Biol. Chem. 267:2947.
  7. Ku, N.-O. and R.F. Mortensen (1993) Cytokine 5:319.
  8. Mold, C. et al. (2002) J. Immunol. 169:7019.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for C-Reactive Protein/CRP Antibody (AF1829)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol CRP
Uniprot