C-Reactive Protein/CRP Antibody [Unconjugated]

Images

 
C-Reactive Protein/CRP was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) at 5 µg/mL overnight at 4 ...read more
Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for C‑Reactive Protein/CRP at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Rat ...read more
Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat C‑Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1744) followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

C-Reactive Protein/CRP Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat C-Reactive Protein/CRP
His20-Ser230
Accession # P48199
Specificity
Detects rat C‑Reactive Protein/CRP in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse CRP is observed and 10% cross-reactivity with recombinant human CRP is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1744 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C-Reactive Protein/CRP Antibody [Unconjugated]

  • C-Reactive Protein
  • C-reactive protein, pentraxin-related
  • CRP
  • MGC88244
  • pentraxin 1
  • PTX1MGC149895

Background

C-Reactive Protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. The rat CRP gene encodes a 230 amino acid (aa) precursor with a signal peptide of 19 aa and the mature polypeptide of 211 aa. Rat CRP shares 64% and 70% aa sequence homology with human and mouse CRP respectively. Human, mouse and rabbit CRP are non-glycosylated proteins, and the units are non-covalently linked to form the pentameter. In contrast, rat CRP is a glycoprotein and contains a covalently linked dimer in the pentameter. CRP exhibits Ca++-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, the membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phagocytotic responses. In addition to phagocytosis, CRP also induces the production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF-alpha . In human and rabbits, CRP is an important acute-phase protein that plays a role in the first line of host innate defense. The level of plasma CRP at basal conditions in human and rabbits is very low, and can increase 1,000-fold within 24‑48 hours in response to infection, inflammation or tissue damage. In rats, CRP exists at a high level at basal conditions and only increases about 2-fold in response to injury. CRP is not a typical acute-phase protein in rat and is a minor component in response to injury. In mice, CRP is expressed at very low levels and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is an acute phase serum protein in mice.

  1. Mohammad, R. et al. (1992) J. Biol. Chem. 267:2947.
  2. Sambasivam, H. et al. (1993) J. Biol. Chem. 268:10007.
  3. Padilla, N.D. et al. (2003) Immunology 109:564.
  4. Volanakis, J.E. (2001) Molecular Immunology 38:189.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CRP
Uniprot