C-Reactive Protein/CRP Antibody (C6cc) - BSA Free

• Reactivity: Hu, Mu
• Applications: ELISA, IHC, AP
• Clone: C6cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

C-Reactive Protein/CRP Antibody (C6cc) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human CRP.
• Localization: Secreted
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoaffinity Purification
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
• Application Notes: Human C-reactive protein immunodetection in direct ELISA, high sensitivity sandwich immunoassay, competitive immunoassays, turbidimetric assays, immunoaffinity purification, immunohistochemistry. Recommended pair for hsCRP sandwich immunoassay (capture-detection): NB600-1333 - NB200-442.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27323407).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for C-Reactive Protein/CRP Antibody (C6cc) - BSA Free

• C-Reactive Protein
• C-reactive protein, pentraxin-related
• CRP
• MGC88244
• pentraxin 1
• PTX1MGC149895

Background

C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C-Reactive Protein/CRP Antibody (NB200-442)(1)

Publication using NB200-442

• Chen Meng-Chuan, Hsu Wen-Lin, Hwang Pa-An et al. Combined administRation of fucoidan amelioRates tumor and chemotherapy-induced skeletal muscle atrophy in bladder cancer-bearing mice. Oncotarget 2016-01-01 [PMID: 27323407] (IF/IHC, Mouse)

FAQs for C-Reactive Protein/CRP Antibody (NB200-442). (Showing 1 - 3 of 3 FAQ).

1. Do you have an assigned shelf-life for product catalog # NB200-442?
   • We guarantee all of our antibodies for up to 6 months from the date you receive the vial. The product should be viable after this date however if storage conditions are followed and the product is not frozen and thawed as this can denature the antibody.
2. Do you know if this antibody is utilized in any FDA cleared bioassays? 
   • We have no knowledge that this antibody has ever been used in FDA cleared bioassays.
3. We are looking to purchase some antibodies against c-reactive protein (preferably antibody clones c6 and c2). For our purpose, we are looking for antibodies that are preferably optimized for, or at least have reported use with, human serum samples, because we are wary of the serum matrix effect on the functioning of the antibody. Do you have any recommendations for these requirements?
   • For human c-reactive protein, we currently sell 18 antibodies which recognise the human protein. Of these, NB110-55637 shows a Western blot image using a human serum sample, while NBP1-87183 and NBP1-87184 have Western blot images generated using human plasma. Clone C2 has catalogue number NB100-73033 and clone C6 has catalogue number NB200-442.

Bioinformatics

• Gene Symbol: CRP
• Entrez:
  • 1401(Human)
• Uniprot:
  • P02741()