c-Myb Antibody

Images

 
Western Blot: c-Myb Antibody [NB100-61662] - Western blot analysis of c-Myb antibody using NB100-61662 at 0.01ug/ml in Human Spleen lysate (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Dilution: 4ug/ml
Western Blot: c-Myb Antibody [NB100-61662] - (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662] - (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with EDTA buffer pH 9, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

c-Myb Antibody Summary

Immunogen
Peptide with sequence C-QRHYNDEDPEKEKR corresponding to internal region according to NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1.
Epitope
QRHYNDEDPEKEKR
Specificity
This antibody is expected to recognize isoforms 1-7 only (Numbering from NCBI RefSeq). (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Approx. 70 kDa band observed in human colon lysates (calculated MW of 72.3 kDa band according to NP_005366.2). IHC-P: kidney shows nuclear staining in all cells.
Positive Control
c-Myb Lysate (NBL1-13410)
Publications
Read Publication using NB100-61662.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Myb Antibody

  • c-myb protein (140 AA)
  • Cmyb
  • c-myb
  • c-myb_CDS
  • c-myb10A_CDS
  • c-myb13A_CDS
  • c-myb14A_CDS
  • c-myb8B_CDS
  • efg
  • Proto-oncogene c-Myb
  • transcriptional activator Myb
  • v-myb avian myeloblastosis viral oncogene homolog
  • v-myb myeloblastosis viral oncogene homolog (avian)

Background

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Myb Antibody (NB100-61662)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYB
Entrez
Uniprot