c-jun [p Thr91] Antibody (SR1729) - BSA Free

Western Blot: c-jun [p Thr 91] Antibody (SR1729) [NBP3-21560] - Western blot analysis using NBP3-21560 in human brain lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clone: SR1729
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

c-jun [p Thr91] Antibody (SR1729) - BSA Free Summary

• Description: Novus Biologicals Rabbit c-jun [p Thr91] Antibody (SR1729) - BSA Free (NBP3-21560) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-c-Jun [Thr91] (Uniprot #: P05412)
• Modification: p Thr91
• Specificity: Detects endogenous levels of phospho-c-Jun [Thr91]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: JUN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:20
  • Western Blot 1:1000-1:2000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for c-jun [p Thr91] Antibody (SR1729) - BSA Free

• Activator protein 1
• AP1
• AP-1
• cJun
• c-Jun
• enhancer-binding protein AP1
• Jun activation domain binding protein
• jun oncogene
• jun proto-oncogene
• JUN
• Proto-oncogene c-Jun
• transcription factor AP-1
• V-jun avian sarcoma virus 17 oncogene homologp39
• v-jun sarcoma virus 17 oncogene homolog (avian)
• v-jun sarcoma virus 17 oncogene homolog

Background

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: JUN