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c-Abl Antibody

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Western Blot: c Abl Antibody [NB110-81723] - Detection of C-ABL in K562 whole cell lysate using NB110-81723.
Western Blot: c Abl Antibody [NB110-81723] - Detection of C-ABL in K562 whole cell lysate using NB110-81723.
Immunocytochemistry/ Immunofluorescence: c Abl Antibody [NB110-81723] - ICC/IF staining of c-Abl in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
DyLight 405
NB110-81723V
FITC
NB110-81723F
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c-Abl Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human c Abl (within residues 700-800). [Swiss-Prot# P00519]
Localization
Cytoplasm, cytoskeleton. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
Application Notes
This c Abl antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~120 kDa. In ICC/IF cytoplasmic staining is observed in untreated cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
K-562 Whole Cell Lysate
c-Abl Overexpression Lysate
c-Abl Knockout HeLa Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Abl Antibody

  • Abelson murine leukemia viral oncogene homolog 1
  • ABL
  • ABL1
  • bcr/abl
  • BCR-ABL1
  • c-abl oncogene 1, non-receptor tyrosine kinase
  • c-abl oncogene 1, receptor tyrosine kinase
  • cAbl
  • c-Abl
  • EC 2.7.10
  • EC 2.7.10.2
  • JTK7
  • JTK7bcr/abl
  • p150bcr/c-abl oncogene protein
  • Proto-oncogene c-Abl
  • proto-oncogene tyrosine-protein kinase ABL1
  • tyrosine-protein kinase ABL1
  • v-abl Abelson murine leukemia viral oncogene homolog 1
  • v-abl

Background

c-ABL regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. It localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Activity of the c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. c-ABL also potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of RUNX1+RUNX2+RUNX3 on Jurkat cells lysates using anti-RUNX1+RUNX2+RUNX3 antibody at 1/1,000 dilution.
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Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of paraffin-embedded mouse testis tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(8 Publications)
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ERK2 Antibody
(191801) [Unconjugate
MAB1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERK2 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ERK2 was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast adipose-like cell line using Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for ERK2 at approximately 43 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
c-Abl Antibody
NB110-81723
c-Abl Antibody
Species: Hu, Mu
Applications: WB, ICC/IF

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Control Lysate(s)

K-562 Whole Cell Lysate
c-Abl Overexpression Lysate
c-Abl Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 NB110-81723V
FITC NB110-81723F
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ABL1
Entrez
Uniprot