c-Abl Antibody

Images

 
Western Blot: c Abl Antibody [NB110-81723] - Detection of C-ABL in K562 whole cell lysate using NB110-81723.
Immunocytochemistry/ Immunofluorescence: c Abl Antibody [NB110-81723] - ICC/IF staining of c-Abl in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

c-Abl Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human c Abl (within residues 700-800). [Swiss-Prot# P00519]
Localization
Cytoplasm, cytoskeleton. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
Application Notes
This c Abl antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~120 kDa. In ICC/IF cytoplasmic staining is observed in untreated cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
K-562 Whole Cell Lysate
c-Abl Overexpression Lysate
c-Abl Knockout HeLa Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Abl Antibody

  • Abelson murine leukemia viral oncogene homolog 1
  • ABL
  • ABL1
  • bcr/abl
  • BCR-ABL1
  • c-abl oncogene 1, non-receptor tyrosine kinase
  • c-abl oncogene 1, receptor tyrosine kinase
  • cAbl
  • c-Abl
  • EC 2.7.10
  • EC 2.7.10.2
  • JTK7
  • JTK7bcr/abl
  • p150bcr/c-abl oncogene protein
  • Proto-oncogene c-Abl
  • proto-oncogene tyrosine-protein kinase ABL1
  • tyrosine-protein kinase ABL1
  • v-abl Abelson murine leukemia viral oncogene homolog 1
  • v-abl

Background

c-ABL regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. It localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Activity of the c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. c-ABL also potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for c-Abl Antibody (NB110-81723): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 NB110-81723V
FITC NB110-81723F

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Bioinformatics

Gene Symbol ABL1
Entrez
Uniprot