Butyrylcholinesterase/BCHE Antibody - BSA Free

Immunohistochemistry-Paraffin: Butyrylcholinesterase/BCHE Antibody [NBP1-85633] - Staining of human liver shows cytoplasmic positivity in hepatocytes.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Butyrylcholinesterase/BCHE Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEILLNEAFVVPYGTPLSVNFGPTVD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCHE
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Western Blot reported in scientific literature (PMID:31765413).
• Control Peptide:

  Butyrylcholinesterase/BCHE Protein (NBP1-85633PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31765413).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Butyrylcholinesterase/BCHE Antibody - BSA Free

• Acylcholine acylhydrolase
• BCHE
• Butyrylcholine esterase
• Butyrylcholinesterase
• CHE1
• CHE1cholinesterase
• Choline esterase II
• cholinesterase 1
• E1
• EC 3.1.1.8
• Pseudocholinesterase

Background

Butyrylcholinesterase (BtChE, EC 3.1.1.8.) is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. Butyrylcholinesterase is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits, each with molecular mass of 90 kDa (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Butyrylcholinesterase/BCHE Antibody (NBP1-85633)(2)

Publications using NBP1-85633

• Gupta V, Cadieux CL, McMenamin D, et al. Adeno-associated virus-mediated expression of human butyrylcholinesterase to treat organophosphate poisoning PLoS ONE 2019-11-25 [PMID: 31765413] (WB, Mouse)
• Kato BS, Nicholson G, Neiman M et al. Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model. Proteome Sci 2011-11-17 [PMID: 22093360]

Bioinformatics

• Gene Symbol: BCHE