BubR1 Antibody - C-terminus - BSA Free

Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.

• Reactivity: Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: BSA Free

BubR1 Antibody - C-terminus - BSA Free Summary

• Description: Novus Biologicals Sheep BubR1 Antibody - C-terminus - BSA Free (NBP3-30805) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein corresponding to amino acids 883-1052 of the mouse BubR1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: BUB1B
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation : 1-10 ug/ml
  • Western Blot : 1-10 ug/ml
• Application Notes: Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot. Detects a 130 kDa band..

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.08% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for BubR1 Antibody - C-terminus - BSA Free

• Bub1A
• BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
• budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• EC 2.7.11.1
• hBUBR1
• MAD3/BUB1-related protein kinase
• MAD3LBUB1beta
• Mitotic checkpoint kinase MAD3L
• Protein SSK1
• SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB1B