Brorin/VWC2 Antibody [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

Brorin/VWC2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Brorin/VWC2
  Ser28-Met324
  Accession # Q8C8N3
• Specificity: Detects mouse Brorin/VWC2 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human Brorin/VWC2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brorin/VWC2 Antibody [Alexa Fluor® 488]

• Brain-specific chordin-like protein
• Brain-Specific Chordin-Like
• Brorin
• PSST739
• UNQ739
• Von Willebrand Factor C Domain Containing 2
• Von Willebrand Factor C Domain-Containing Protein 2
• VWC2

Background

Mouse VWC2 (von Willebrand Factor C domain-containing protein 2; also Brorin and cradin) is a secreted, 35 kDa (predicted) member of the chordin family of proteins. It is expressed in the choroid plexus and by neurons in multiple sites, and serves to sequester BMP family proteins such as BMP-2 and BMP-6. BMP antagonism is suggested to generate neurons from neural precursor cells at the expense of astrocytes. Mouse VWC2 precursor is 324 amino acids (aa) in length. It contains a 27 aa signal sequence, one poly-Ala region (aa 124-129), and two von Willebrand factor C domains (aa 152-211 and 215-273) that likely serve as BMP interaction sites. Over aa 28-324, mouse VWC2 shares 90% aa identity with human VWC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.