Crossveinless-2/CV-2/BMPER Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Crossveinless-2/CV-2/BMPER Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Crossveinless-2/CV-2 (R&D Systems, Catalog # 2299-CV)
Ala39-Arg685
Accession # AAH66153
Specificity
Detects mouse Crossveinless-2/CV-2 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human CV-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BMPER
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2299 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Crossveinless-2/CV-2/BMPER Antibody

  • BMP binding endothelial regulator
  • BMP-binding endothelial regulator precursor protein
  • BMP-binding endothelial regulator protein
  • BMPER
  • Bone morphogenetic protein-binding endothelial cell precursor-derived regulator
  • CRIM3
  • crossveinless 2
  • Crossveinless-2
  • Cv2
  • CV-2
  • hCV2
  • KIAA1965
  • Protein crossveinless-2

Background

Crossveinless-2 (CV-2), also known as bone morphogenetic protein-binding endothelial cell precursor-derived regulator (BMPER), is a secreted chordin-like protein that modulates the BMP signaling pathway (1‑3). Mouse CV-2 is synthesized as a 685 amino acid (aa) residue precursor protein with a putative 39 aa signal peptide, five tandem chordin-like cysteine-rich (CR) domains, a partial von Willebrand factor type D domain (vWD), and a carboxyl trypsin inhibitor-like cysteine-rich domain (TIL) (1, 2, 4). Secreted CV-2 is reported to be proteolytically cleaved to generate two fragments that are disulfide-linked (1, 2). The GDPH sequence is conserved in CV-2 from other species. It is also found in multiple proteins that undergo a similar type of cleavage (5). Mouse CV-2 message is detected in many tissues, with the highest expression detected in the heart, lungs, and skin (2). It is also expressed in flk-1+ endothelial cell precursors and in primary chondrocytes (2). During embryonic development, CV-2 is expressed in the dorsal midline, regions of the telencephalon, migrating cells of the branchial neural crest and endothelial cells in the yolk sac (2). Mouse CV-2 shares 92% and 34% aa sequence identity with the human and Drosophila homologs, respectively (1, 4). Results from biochemical experiments using recombinant CV-2 show that CV-2 directly interacts with BMP-2, -4, and -6 to antagonize BMP signaling, which can regulate a wide range of differentiation processes (1, 2). In contrast, genetic data from Drosophila suggest that CV-2 potentiates BMP-signaling (6). It is possible that like TSG, CV-2 can positively and negatively modulate BMP signal transduction depending on the cell context (7).

  1. Binnerts, M.E. et al. (2004) Biochem Biophys Res Commun. 315:272.
  2. Moser, M. et al. (2003) Mol Cell Biol. 23:5664.
  3. Garcia-Abreu, J. et al. (2002) Gene 287:39.
  4. Coffinier, C. et al. (2002) Mech Dev. 119:S179.
  5. Lidell, M.E. et al. (2003) J. Biol. Chem. 278:13944.
  6. Conley, C.A. et al. (2000) Development 127:3947.
  7. Kamimura, M. et al. (2004) Developmental Dynamics 230:434.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Crossveinless-2/CV-2/BMPER Antibody (AF2299)(5)

We have publications tested in 2 confirmed species: Mouse, Zebrafish.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


Filter By Application
IHC
(2)
IHC-Fr
(1)
Western Blot
(2)
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Mouse
(4)
Zebrafish
(1)
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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2299

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Bioinformatics Tool for Crossveinless-2/CV-2/BMPER Antibody (AF2299)

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Diseases for Crossveinless-2/CV-2/BMPER Antibody (AF2299)

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Bioinformatics

Gene Symbol BMPER
Uniprot