Brorin/VWC2 Antibody (615609) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: IHC
• Clone: 615609
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Brorin/VWC2 Antibody (615609) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Brorin/VWC2
  Ser28-Met325
  Accession # Q2TAL6
• Specificity: Detects human Brorin/VWC2 in direct ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant mouse Brorin/VWC2 and no cross‑reactivity with recombinant human (rh) vWF-A2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brorin/VWC2 Antibody (615609) [Alexa Fluor® 700]

• Brain-specific chordin-like protein
• Brain-Specific Chordin-Like
• Brorin
• PSST739
• UNQ739
• Von Willebrand Factor C Domain Containing 2
• Von Willebrand Factor C Domain-Containing Protein 2
• VWC2

Background

Brorin (brain-specific chordin-like protein), also called VWC2, is an ~46 kDa glycoprotein that is a member of the Chordin family of secreted BMP regulators (1‑3). The human Brorin cDNA encodes 325 amino acids (aa) including a 27 aa signal sequence and a 298 aa secreted mature protein with two VWFC (von Willebrand factor C) domains. These domains contain a pattern of 10 cysteine residues that is conserved in other family members, with the remaining aa sequence sharing little identity (1). Human Brorin shares 90%, 91% and 95% aa sequence identity with mouse, rat and equine Brorin, respectively. It also shares aa identity with VWC2L (Brorin-like) of 37% overall and 62% within the VWFC domains (4). Brorin is predominantly expressed in embryonic and adult neural tissues in the mouse (1). Expression of Brorin mRNA is concentrated in neurons within the diencephalon and medulla oblongata, but is not detected in the developing cerebral cortex (1). Brorin binds and antagonizes BMPs, interacting via the VWFC domains (1‑3). It promotes neurogenesis in mouse neural precursors (1). Knockdown of Brorin in zebrafish embryos results in morphological abnormalities in the brain and eye (1, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q2TAL6(Human)