Brk Antibody (2H12B8)

Western Blot: Brk Antibody (2H12B8) [NB110-60537] - Analysis using PTK6 mouse mAb against Hela (1), A549 (2) and MCF-7 (3) cell lysate.

Western Blot: Mouse Monoclonal Brk Antibody (2H12B8) [NB110-60537] - Whole cell lysates from MDA-MB-231 cells that were exposed to 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. Brk Antibody (NB110-60537) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2H12B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Brk Antibody (2H12B8) Summary

• Description: Novus Biologicals Mouse Brk Antibody (2H12B8) (NB110-60537) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Brk expressed in E. Coli.
• Specificity: PTK6 (2H12B8)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTK6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Brk Antibody (2H12B8)

• Breast tumor kinase
• Brk
• BRKprotein-tyrosine kinase 6
• EC 2.7.10
• EC 2.7.10.2
• FLJ42088
• protein-tyrosine kinase BRK
• PTK6 protein tyrosine kinase 6
• PTK6
• Sik
• Tksk
• Tyrosine-protein kinase BRK

Background

PTK6 (protein tyrosine kinase 6, BRK or FLJ42088),with 451-amino acid protein (about 51kDa), encods a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphory-lation. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. And Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes, but not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Over expression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Brk Antibody (NB110-60537) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Yongkang Yang WB Human 11/27/2024

Summary

• Application: Western Blot
• Sample Tested: human MDAMB-231 whole cell lysate
• Species: Human
• Lot: 150813

Bioinformatics

• Gene Symbol: PTK6
• Entrez:
  • 5753(Human)