Brk Antibody (2F11) - Azide and BSA Free

Immunohistochemistry-Paraffin: Brk Antibody (2F11) [H00005753-M01] - Analysis of monoclonal antibody to PTK6 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 2F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Brk Antibody (2F11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PTK6 (NP_005966, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YLSHDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT
• Specificity: PTK6 - PTK6 protein tyrosine kinase 6 (2F11)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTK6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Brk Antibody (2F11) - Azide and BSA Free

• Breast tumor kinase
• Brk
• BRKprotein-tyrosine kinase 6
• EC 2.7.10
• EC 2.7.10.2
• FLJ42088
• protein-tyrosine kinase BRK
• PTK6 protein tyrosine kinase 6
• PTK6
• Sik
• Tksk
• Tyrosine-protein kinase BRK

Background

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brk Antibody (H00005753-M01)(2)

Publications using H00005753-M01

• Falkenberg N, Anastasov N, Bashkueva K et al. Additive impact of HER2-/PTK6-RNAi on interactions with HER3 or IGF-1R leads to reduced breast cancer progression in vivo. Molecular Oncology. 2014-01-01 [PMID: 25241146]
• Akl MR, Ayoub NM, Mohyeldin MM et al. Olive Phenolics as c-Met Inhibitors: (-)-Oleocanthal Attenuates Cell Proliferation, Invasiveness, and Tumor Growth in Breast Cancer Models. PLoS One. 2014-05-21 [PMID: 24849787]

Bioinformatics

• Gene Symbol: PTK6
• Entrez:
  • 5753(Human)
• Uniprot:
  • NP_005966(Human)