BRIP1/FANCJ Antibody

Product Discontinued

BRIP1/FANCJ Antibody Summary

• Immunogen: A synthetic peptide within the extreme C-terminus of human FANCJ. [Swiss-Prot# Q9BX63]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BRIP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:200
• Application Notes: Use in immunoblotting reported in scientific literature (PMID 25070891). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.01% Sodium Azide
• Purity: Unpurified

Alternate Names for BRIP1/FANCJ Antibody

• ATP-dependent RNA helicase BRIP1
• BACH1
• BACH1BRCA1/BRCA2-associated helicase 1
• BRCA1 interacting protein C-terminal helicase 1
• BRCA1-associated C-terminal helicase 1
• BRCA1-binding helicase-like protein BACH1
• BRCA1-interacting protein 1
• BRCA1-interacting protein C-terminal helicase 1
• BRIP1
• EC 3.6.1
• EC 3.6.4.13
• FACJ
• FANCJ
• FANCJFanconi anemia group J protein
• FLJ90232
• MGC126521
• MGC126523
• OF
• Protein FACJ

Background

FANCJ (Fanconi anemia group J protein) was originally identified as a protein which binds BRCT domains of BRCA1 and was named BACH1 (BRCA1-Associated C-terminal Helicase1) or BRIP1 (BRCA1 Interacting Protein C-terminal Helicase1), and after its identification as a component of Fanconi anemia pathway, it was renamed as FANCJ. It is expressed ubiquitously with highest levels in testes and sub-cellularly, it localizes to the nucleus. FANCJ is a DNA-dependent ATPase with 5' to 3' DNA helicase activity which is essential for genetic and structural integrity of genome. FANCJ acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination, and is implicated in repairing DNA double-strand breaks through homologous recombination in FANCJ-BRCA1 interactions dependent manner. Cell-cycle regulated phosphorylation of FANCJ is essential for BRCA1 interaction and 4Fe-4S iron-sulfur-binding is required for its helicase activity. The interaction of FANCJ with proteins including MutLalpha complex and BRCA1, suggests its importance in other pathways of DNA repair and in diseases such as colon and breast cancer. Defective FANCJ causes chromosome instability disorder Fanconi anemia complementation group J.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BRIP1/FANCJ Antibody (NB100-416)(14)

Publications using NB100-416

• Alghoul E, Basbous J, Constantinou A An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells STAR Protocols 2021-09-01 [PMID: 34377994] (IP)
• Frattini C, Promonet A, Alghoul E, et al. TopBP1 assembles nuclear condensates to switch on ATR signaling Molecular cell 2021-01-16 [PMID: 33503405]
• Brannvoll A, Xue X, Kwon Y et al. The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells Cell Rep 2020-07-07 [PMID: 32640219] (WB, Human)
• Rossi F, Noren H, Sarria L, et al. SMC5/6 acts jointly with Fanconi anemia factors to support DNA repair and genome stability EMBO Rep. 2019-12-23 [PMID: 31867888]
• Popp I, Punekar M, Telford N et al. Fanconi anemia with sun-sensitivity caused by a Xeroderma pigmentosum-associated missense mutation in XPF BMC Med. Genet. 2018-01-11 [PMID: 29325523] (WB, Human)
• Bermudez-Hernandez K, Keegan S, Whelan DR et al. A Method for Quantifying Molecular Interactions Using Stochastic Modelling and Super-Resolution Microscopy Sci Rep. 2017-11-01 [PMID: 29093506] (Human)
• Romick-Rosendale LE, Hoskins EE, Privette Vinnedge LM et al. Defects in the Fanconi anemia pathway in head and neck cancer cells stimulate tumor cell invasion through DNA-PK and Rac1 signaling. Clin. Cancer Res. 2015-11-24 [PMID: 26603260]
• Aarts M, Bajrami I, Herrera-Abreu MT et al. Functional genetic screen identifies increased sensitivity to WEE1 inhibition in cells with defects in Fanconi Anaemia and HR pathways Mol. Cancer Ther. 2015-02-11 [PMID: 25673822] (WB, Human)
• Knies K, Schuster B, Ameziane N et al. Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges PLoS One 2012-01-01 [PMID: 23285130] (WB, Human)
• Sakasai R, Sakai A, Iimori M et al. CtIP- and ATR-dependent FANCJ phosphorylation in response to DNA strand breaks mediated by DNA replication. Genes Cells 2012-12-01 [PMID: 23157317] (WB, Human)
• Nakanishi R, Kitao H, Fujinaka Y, Yamashita N, Iimori M, Tokunaga E, Yamashita N, Morita M, Kakeji Y, Maehara Y. FANCJ Expression Predicts the Response to 5-Fluorouracil-Based Chemotherapy in MLH1-Proficient Colorectal Cancer. Ann Surg Oncol. 2012-04-12 [PMID: 22526901] (IF/IHC, WB, Human)
• Vaz F, Hanenberg H, Schuster B et al. Mutation of the RAD51C gene in a Fanconi anemia-like disorder. Nat Genet. 2010-05-01 [PMID: 20400963] (WB, Human)
• Duan SZ, Ivashchenko CY, Russell MW, Milstone DS, Mortensen RM. Cardiomyocyte-specific knockout and agonist of peroxisome proliferator-activated receptor-gamma both induce cardiac hypertrophy in mice. Circ Res;97(4):372-9. 2005-08-19 [PMID: 16051889] (WB, Mouse)
• Cantor, S et al. BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function. Cell;105(1):149-60. 2001-04-06 [PMID: 11301010] (WB, IP, Human)

Reviews for BRIP1/FANCJ Antibody (NB100-416) (4)

Average Rating: 4.5

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Human.

reviewed by: Kerstin Knies WB Human 02/28/2014

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate of human fibroblasts
• Species: Human

reviewed by: Ryota Nakanishi IF Human 06/06/2012

Summary

• Application: Immunofluorescence
• Sample Tested: colon and rectum
• Species: Human
• Lot: 100-416

reviewed by: Verified Customer WB Human 03/05/2010

Summary

• Application: Western Blot
• Sample Tested: Human cell lines, Sample Amount: 20ug total protein
• Species: Human
• Lot: A

reviewed by: Verified Customer WB Human 03/04/2009

Summary

• Application: Western Blot
• Sample Tested: human fibroblasts, Sample Amount: 20ug
• Species: Human
• Lot: A

FAQs for BRIP1/FANCJ Antibody (NB100-416). (Showing 1 - 1 of 1 FAQs).

1. Have you had any reports of attempted use of NB100-416 in ChIP or has it been tested only in traditional IP?
   • Our FANCJ Antibody (NB100-416) has not yet been tested in Chromatin Immunoprecipitation, therefore we cannot guarantee it’s use for this application. However, it has been tested in traditional IP. We do have a general ChIP protocol which you may view here. If you would be interested in testing this novel ChIP application, please take a look at our Innovators Reward Program.

Bioinformatics

• Gene Symbol: BRIP1
• Entrez:
  • 83990(Human)
• Uniprot:
  • Q9BX63()