BRIP1/FANCJ Antibody

Images

 
Western Blot: BRIP1/FANCJ Antibody [NB100-416] - Analysis on MCF7, HeLa, 293T and SKOV3 crude cell lysates.
Immunohistochemistry: BRIP1/FANCJ Antibody [NB100-416] - Analysis of BRIP1/FANCJ in human colon and rectum tissues. Image courtesy of an anonymous customer review.
Western Blot: BRIP1/FANCJ Antibody [NB100-416] - BRIP1/FANCJ detected in cell lysates using NB 100-416. Lane 1: MCF-7 lysate, Lane 2: HeLa lysate, Lane 3: 293 lysate and Lane 4: SKOV3 lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BRIP1/FANCJ Antibody Summary

Immunogen
A synthetic peptide within the extreme C-terminus of human FANCJ. [Swiss-Prot# Q9BX63]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRIP1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:200
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This FANCJ antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot, where a band can be seen at ~130 kDa. NB100-416 has also been successfully used for Immunofluorescence as reported by a customer review.Use in immunoblotting reported in scientific literature (PMID 25070891) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB100-416 in the following applications:

Publications
Read Publications using
NB100-416 in the following applications:

  • IHC
    1 publication
  • IP
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.01% Sodium Azide
Purity
Unpurified

Alternate Names for BRIP1/FANCJ Antibody

  • ATP-dependent RNA helicase BRIP1
  • BACH1
  • BACH1BRCA1/BRCA2-associated helicase 1
  • BRCA1 interacting protein C-terminal helicase 1
  • BRCA1-associated C-terminal helicase 1
  • BRCA1-binding helicase-like protein BACH1
  • BRCA1-interacting protein 1
  • BRCA1-interacting protein C-terminal helicase 1
  • BRIP1
  • EC 3.6.1
  • EC 3.6.4.13
  • FACJ
  • FANCJ
  • FANCJFanconi anemia group J protein
  • FLJ90232
  • MGC126521
  • MGC126523
  • OF
  • Protein FACJ

Background

FANCJ (Fanconi anemia group J protein) was originally identified as a protein which binds BRCT domains of BRCA1 and was named BACH1 (BRCA1-Associated C-terminal Helicase1) or BRIP1 (BRCA1 Interacting Protein C-terminal Helicase1), and after its identification as a component of Fanconi anemia pathway, it was renamed as FANCJ. It is expressed ubiquitously with highest levels in testes and sub-cellularly, it localizes to the nucleus. FANCJ is a DNA-dependent ATPase with 5' to 3' DNA helicase activity which is essential for genetic and structural integrity of genome. FANCJ acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination, and is implicated in repairing DNA double-strand breaks through homologous recombination in FANCJ-BRCA1 interactions dependent manner. Cell-cycle regulated phosphorylation of FANCJ is essential for BRCA1 interaction and 4Fe-4S iron-sulfur-binding is required for its helicase activity. The interaction of FANCJ with proteins including MutLalpha complex and BRCA1, suggests its importance in other pathways of DNA repair and in diseases such as colon and breast cancer. Defective FANCJ causes chromosome instability disorder Fanconi anemia complementation group J.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRIP1/FANCJ Antibody (NB100-416)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IP, WB.


Filter By Application
IHC
(1)
IP
(1)
WB
(7)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-416 Applications Species
Romick-Rosendale LE, Hoskins EE, Privette Vinnedge LM et al. Defects in the Fanconi anemia pathway in head and neck cancer cells stimulate tumor cell invasion through DNA-PK and Rac1 signaling. Clin. Cancer Res. 2015 Nov 24 [PMID: 26603260]
Aarts M, Bajrami I, Herrera-Abreu MT et al. Functional genetic screen identifies increased sensitivity to WEE1 inhibition in cells with defects in Fanconi Anaemia and HR pathways Mol. Cancer Ther. 2015 Feb 11 [PMID: 25673822] (WB, Human)

Details:
BRIP1 antibody used for WB analysis of human cell lysates that were transfected with BRIP1 siRNA (Figure 1).
WB Human
Knies K, Schuster B, Ameziane N et al. Genotyping of fanconi anemia patients by whole exome sequencing: advantages and challenges PLoS One 2012 [PMID: 23285130] (WB, Human) WB Human
Sakasai R, Sakai A, Iimori M et al. CtIP- and ATR-dependent FANCJ phosphorylation in response to DNA strand breaks mediated by DNA replication Genes Cells 2012 Dec [PMID: 23157317] (WB, Human) WB Human
Nakanishi R, Kitao H, Fujinaka Y, Yamashita N, Iimori M, Tokunaga E, Yamashita N, Morita M, Kakeji Y, Maehara Y. FANCJ Expression Predicts the Response to 5-Fluorouracil-Based Chemotherapy in MLH1-Proficient Colorectal Cancer. Ann Surg Oncol. 2012 Apr 12. [PMID: 22526901] (IHC, WB, Human) IHC, WB Human
Vaz F, Hanenberg H, Schuster B et al. Mutation of the RAD51C gene in a Fanconi anemia-like disorder. Nat Genet. 2010 May [PMID: 20400963] (WB, Human) WB Human
Duan SZ, Ivashchenko CY, Russell MW, Milstone DS, Mortensen RM. Cardiomyocyte-specific knockout and agonist of peroxisome proliferator-activated receptor-gamma both induce cardiac hypertrophy in mice. Circ Res;97(4):372-9. 2005 Aug 19. [PMID: 16051889] (WB, Mouse) WB Mouse
Cantor, S et al. BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function. Cell;105(1):149-60. 2001 Apr 6. [PMID: 11301010] (WB, IP, Human) WB, IP Human

Reviews for BRIP1/FANCJ Antibody (NB100-416) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.
We have 4 reviews tested in 2 application: WB, IF.

Reviews using NB100-416:
Filter by Applications
WB
(3)
IF
(1)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Kerstin Knies
WB Human 02/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate of human fibroblasts
SpeciesHuman

Blocking

Blocking DetailsBlocking milk 5% in PBS-T over night at 4°C

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptionrabbit anti mouse IgG HRP
Secondary Concentration1:10000

Details

Detection NotesHRP Substratet of Millipore, exposure time 2 min.
Immunofluorescence BRIP1/FANCJ NB100-416
Enlarge
reviewed by:
Ryota Nakanishi
IF Human 06/06/2012
View

Summary

ApplicationImmunofluorescence
Sample Testedcolon and rectum
SpeciesHuman
Lot100-416

Blocking

Blocking Detailsskim milk

Primary Anitbody

Dilution Ratio1/200, 4℃, overnight
 
reviewed by:
Anonymous
WB Human 03/05/2010
View

Summary

ApplicationWestern Blot
Sample TestedHuman cell lines, Sample Amount: 20ug total protein
SpeciesHuman
LotA

Blocking

Blocking DetailsBlocking Buffer: 10% TBST with dry milk, Blocking Time: 90 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/2000, Incubation Dilution Buffer: TBST, Incubation Time: 1 hour, Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP conjugated donkey against rabbit polyclonal antibody, Secondary Ab Dilution Ratio: 1/5000

Details

Detection NotesDetection Method: ECL, Exposure Time: 10sec, Positive Control: HeLa cell extract, Negative Control: FANCJ deficient cell extract
 
reviewed by:
Anonymous
WB Human 03/04/2009
View

Summary

ApplicationWestern Blot
Sample Testedhuman fibroblasts, Sample Amount: 20ug
SpeciesHuman
LotA

Blocking

Blocking DetailsBlocking Buffer: 10% TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/2000, Incubation Dilution Buffer: TBST with 5% milk, Incubation Time: 1.5 hours, Incubation Temp: 25 C

Secondary Antibody

Secondary DescriptionSecondary Ab: pAb Goat anti-rabbit IgG HRP, Secondary Ab Dilution Ratio: 1/5000

Details

Detection NotesDetection Method: ECL, Exposure Time: 5 minutes, Specific Bands: 170 kDa

Product General Protocols

View specific protocols for BRIP1/FANCJ Antibody (NB100-416): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BRIP1/FANCJ Antibody (NB100-416). (Showing 1 - 1 of 1 FAQs).

  1. Have you had any reports of attempted use of NB100-416 in ChIP or has it been tested only in traditional IP?
    • Our FANCJ Antibody (NB100-416) has not yet been tested in Chromatin Immunoprecipitation, therefore we cannot guarantee it’s use for this application. However, it has been tested in traditional IP. We do have a general ChIP protocol which you may view here. If you would be interested in testing this novel ChIP application, please take a look at our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BRIP1/FANCJ Antibody (NB100-416)

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Diseases for BRIP1/FANCJ Antibody (NB100-416)

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Pathways for BRIP1/FANCJ Antibody (NB100-416)

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PTMs for BRIP1/FANCJ Antibody (NB100-416)

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Research Areas for BRIP1/FANCJ Antibody (NB100-416)

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Blogs on BRIP1/FANCJ

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

 
Kerstin Knies
02/28/2014
Application: WB
Species: Human

Ryota Nakanishi
06/06/2012
Application: IF
Species: Human

 
Anonymous
03/05/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol BRIP1
Entrez
Uniprot